Hydrogen Sulfide Protects Damage From Methyl Viologen-Mediated Oxidative Stress by Improving Gas Exchange, Fluorescence Kinetics of Photosystem II, and Antioxidant System in Arabidopsis thaliana

Basit Öğe Kaydı
dc.contributor.authorOzfidan-Konakci, Ceyda
dc.contributor.authorYildiztugay, Evren
dc.contributor.authorArikan, Busra
dc.contributor.authorElbasan, Fevzi
dc.contributor.authorAlp, Fatma Nur
dc.contributor.authorKucukoduk, Mustafa
dc.date.accessioned2024-02-23T13:43:43Z
dc.date.available2024-02-23T13:43:43Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractConsidering the unfavorable impacts of methyl viologen-induced oxidative stress (MV1-2, 50 and 500 mu M) on growth, gas exchange (intercellular CO2 concentration, carbon assimilation rate, stomatal conductance, transpiration rate), the efficiency of PSII photochemistry and gene expressions of proteins related to photosystems, antioxidant capacity, and the content/histochemical staining of reactive oxygen species (ROS) markers, the experiment was conducted to evaluate the possible mechanisms of sodium hydrosulfide hydrate (a hydrogen sulfide donor, 500 mu M NaHS) and its scavenger/inhibitor (hypotaurine, 50 mu M and hydroxylamine, 100 mu M) in Arabidopsis thaliana for 24 h. NaHS alleviated stress-reduced growth (4.2-fold increase for MV2 + NaHS) and improved the gas exchange parameters. NaHS was capable of improving the photosynthetic ability under 50 mu M MV through sustaining photochemical activity in PSII and photochemical conversion efficiency as evident by transcript levels of psbA, psbD, psaA, and psaB. Stress-caused oxidative damage was scavenged by POX (a 90% increase). However, this action was not enough, suggested by increased ROS accumulation, lipid peroxidation (a 165% induction) and lipoxygenase activity (2.4-fold increase), and loss of membrane integrity. Meanwhile, NaHS successfully eliminated these responses against MV, evidenced by weak histochemical staining of ROS and lesser lipid peroxidation and membrane damage. The synchronized activities of both SOD and CAT triggered by NaHS were responsible for decreasing H2O2 content (by 57.4% decrease for MV2 + NaHS) in response to MV stress. After stress exposure, NaHS utilized the ascorbate-glutathione (AsA-GSH) cycle for removing H2O2. Arabidopsis subjected to MV1 plus NaHS exhibited the advanced levels of AsA regeneration (by 15.3% increase) and the redox state of GSH. Interestingly, NaHS under the high MV concentration did not maintain the re-establishment of GSH homeostasis and redox state of GSH in spite of the induced AsA/DHA (dehydroascorbate). NaHS could protect Arabidopsis from oxidative stress, likely by regulating growth, gas exchange, and photosynthetic performance, inducing expression levels of genes associated with photosystems and regulating antioxidant capacity, and redox balance for AsA and GSH.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [120Z867]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Project Number: 120Z867).en_US
dc.identifier.doi10.1007/s00344-022-10612-6
dc.identifier.endpage1050en_US
dc.identifier.issn0721-7595
dc.identifier.issn1435-8107
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85125636644en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1031en_US
dc.identifier.urihttps://doi.org/10.1007/s00344-022-10612-6
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10892
dc.identifier.volume42en_US
dc.identifier.wosWOS:000764532600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Plant Growth Regulationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntioxidant Enzymesen_US
dc.subjectHydrogen Sulfideen_US
dc.subjectMethyl Viologenen_US
dc.subjectPhoto-Oxidative Stressen_US
dc.subjectRedox Stateen_US
dc.titleHydrogen Sulfide Protects Damage From Methyl Viologen-Mediated Oxidative Stress by Improving Gas Exchange, Fluorescence Kinetics of Photosystem II, and Antioxidant System in Arabidopsis thalianaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu