Effect of zinc sulfate supplementation on metallothionein levels in rat heart tissue with induced ischemia-reperfusion injury

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dc.contributor.authorYazgan, Betul
dc.contributor.authorBaltaci, Abdulkerim Kasim
dc.contributor.authorMogulkoc, Rasim
dc.contributor.authorAvunduk, Mustafa Cihat
dc.contributor.authorSahna, Engin
dc.date.accessioned2024-02-23T14:49:09Z
dc.date.available2024-02-23T14:49:09Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractMetallothioneins are remarkable proteins with regard to their role in the regulation of intracellular zinc balance and mediation of the physiological effects of zinc, as well as their antioxidant effects. The objective of the present study is to examine how zinc supplementation impacts metallothionein levels in ischemia-reperfusion injury in rats. The study involved heart tissues obtained from 30 Wistar-Albino adult male rats already used in another project. Experimental animals were equally divided into three groups as follows: Group 1: Control (H-Cont); Group 2: Heart Ischemia/Reperfusion (H-I/R); and Group 3: Zinc supplemented Ischemia/Reperfusion (H/Zn-I/R). Cardiac ischemia-reperfusion procedures were carried out under general anesthesia. In the zinc-supplemented cardiac I/R group (Group 3), the animals were supplemented with 5 mg/kg/day intraperitoneal (i.p.) zinc sulfate per rat for 21 days. At the end of the procedures, all animals were decapitated and heart tissues were collected. The tissues were subjected to immunohistochemical staining procedures using rat metallothionein antibody. The stained preparations were photographed and cells stained with metallothionein were counted at a light microscope to calculate their percentages. The analyzed heart tissue samples of the groups did not have any significant difference in terms of their metallothionein levels. Results obtained from the study indicate that 21-day zinc supplementation does not have a critical effect on metallothionein synthesis in cardiac ischemia-reperfusion. This result may be attributed to the dose of zinc, length of supplementation and/or duration of ischemia-reperfusion.en_US
dc.description.sponsorshipScientific Research Projects Coordinatorship of Selcuk University (SUBAPK) [13102019]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Coordinatorship of Selcuk University (SUBAPK; project no. 13102019).en_US
dc.identifier.endpage3196en_US
dc.identifier.issn2069-5837
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85052229090en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3193en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/18028
dc.identifier.volume8en_US
dc.identifier.wosWOS:000435601200005en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBiointerface Research Applied Chemistryen_US
dc.relation.ispartofBiointerface Research In Applied Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCardiac Ischemia-Reperfusionen_US
dc.subjectZinc Supplementationen_US
dc.subjectMetallothioneinen_US
dc.subjectRaten_US
dc.titleEffect of zinc sulfate supplementation on metallothionein levels in rat heart tissue with induced ischemia-reperfusion injuryen_US
dc.typeArticleen_US

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