A new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assay

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dc.contributor.authorOz, Mustafa Emin
dc.contributor.authorTorlak, Emrah
dc.contributor.authorAvci, Oguzhan
dc.contributor.authorDogan, Muge
dc.contributor.authorUncu, Ali Tevfik
dc.date.accessioned2024-02-23T14:37:26Z
dc.date.available2024-02-23T14:37:26Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractThe present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit of detection obtained was approximately 12 RNA copies per reaction corresponding to a C-T value of 36.79. According to the results, it was observed that the developed in-house method has a specific, sensitive, and powerful detection limit as much as the gold standard test. As a result, it was concluded that the proposed method, which was developed as a more cost-effective and easily optimized analysis, could provide an advantage for detecting SRMV in diagnostic laboratories.en_US
dc.description.sponsorshipTAGEMen_US
dc.description.sponsorshipThis study is part of the master's thesis titled Molecular Differentiation of Vaccine and Field Strains of Peste des Petits Ruminants Virus'' supported by TAGEM.en_US
dc.identifier.doi10.3906/vet-2009-41
dc.identifier.endpage220en_US
dc.identifier.issn1300-0128
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85106226995en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage212en_US
dc.identifier.urihttps://doi.org/10.3906/vet-2009-41
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16103
dc.identifier.volume45en_US
dc.identifier.wosWOS:000642323400003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEvagreenen_US
dc.subjectFusion Glycoproteingeneen_US
dc.subjectLow-Cost Diagnosisen_US
dc.subjectReal-Time Rt-Pcren_US
dc.subjectSrmven_US
dc.titleA new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assayen_US
dc.typeArticleen_US

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