Mutagenic effects of sodium azide on in vitro mutagenesis, polymorphism and genomic instability in wheat (Triticum aestivum L.)

Basit Öğe Kaydı
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorTosun, Metin
dc.contributor.authorHaliloglu, Kamil
dc.date.accessioned2024-02-23T13:59:23Z
dc.date.available2024-02-23T13:59:23Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractIntroduction Breeding studies are commonly conducted to develop new cultivars with high yield levels and improved quality traits. Chemically-induced mutations are used to create genetic variations in wheat genomes. Various physical and chemical mutagens are used to increase frequency of mutations and facilitate the selection processes. Sodium azide (SA) is largely employed to induce mutations of the genes regulating essential traits. Such mutations may also elucidate gene functions of the mutant phenotypes. Present experiments were conducted to investigate potential use of conventional chemical mutagenesis technique through SA for mature embryo culture in wheat. Methods and results Sodium azide mutagenesis was experimented with 4 treatment durations (1, 2, 3 and 4 h) and 5 treatment concentrations (0, 1, 2, 3 and 4 mM). Mature embryos were subjected to experimental treatments to detect optimum doses of mutagenesis and to estimate polymorphism and genomic instability. Primarily, 50% reduction in number of regenerated plants as compared to the control (LD50) was adopted as the optimum dose. Based on LD50 criterion, the optimum value was achieved at 1 h duration of 4 mM SA concentration. Afterwards, inter-primer binding site markers were applied to investigate polymorphism and genomic instability in the regenerated plants. Conclusions Present findings revealed that efficiency of chemical mutagenesis could be improved through the use of molecular technology and such mutations may assist plant breeders in developing high-yield cultivars.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG 113O940]en_US
dc.description.sponsorshipIn vitro mutagenesis section in this study presents partial outcomes of Ph.D. thesis of Aras Turkoglu, supported by the Scientific and Technological Research Council of Turkey (TUBITAK, Project no. TOVAG 113O940).en_US
dc.identifier.doi10.1007/s11033-022-07896-y
dc.identifier.endpage10174en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue11en_US
dc.identifier.pmid36029431en_US
dc.identifier.scopus2-s2.0-85137677797en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage10165en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07896-y
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11163
dc.identifier.volume49en_US
dc.identifier.wosWOS:000846216600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndosperm-Supported Mature Embryoen_US
dc.subjectSodium Azideen_US
dc.subjectIpbsen_US
dc.titleMutagenic effects of sodium azide on in vitro mutagenesis, polymorphism and genomic instability in wheat (Triticum aestivum L.)en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu