Effects of mammalian sex hormones on in vitro organogenesis of common bean (Phaseolus vulgaris L.)

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dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorBalpinar, Ozge
dc.contributor.authorOzturk, Halil Ibrahim
dc.contributor.authorOzkan, Guller
dc.contributor.authorPoczai, Peter
dc.date.accessioned2024-02-23T14:16:44Z
dc.date.available2024-02-23T14:16:44Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractBeans are an important plant species and are one of the most consumed legumes in human nutrition, especially as a protein, vitamin, mineral, and fiber source. Common bean (Phaseolus vulgaris L.) is a plant that also has an important role in natural nitrogen fixation. Currently, in vitro regeneration and micropropagation applications are limited in relation to genetic factors in bean Accordingly, there is great need to optimize micropropagation and tissue culture methods of the bean plant. To date, the effect of mammalian sex hormones (MSH) on in vitro conditions in P. vulgaris L. is poorly understood. This study examined the effects of different types of explants (embryo, hypocotyl, plumule, and radicle), MSH type (progesterone, 17 beta-estradiol, estrone, and testosterone), and MSH concentration (10(-4), 10(-6), 10(-8) and 10(-10) mmol L-1) on the responding explants induction rate (REI), viability of plantlets rate (VPR), shoot proliferation rate (SPR), root proliferation rate (RPR), and callus induction rate (CIR). The effects of mammalian sex hormones, concentrations, explant type, and their interactions were statistically significant (p <= 0.01) in all examined parameters. The best explants were embryo and plumule. Our results showed that the highest REI rate (100%) was recorded when 10(-10) mmol L-1 of all MSH was applied to MS medium using the plumule explant. The highest VPR (100%) was obtained when 10(-10) mmol L-1 of all MSH was applied to MS medium using the plumule explant. The highest root proliferation rates (77.5%) were recorded in MS medium supplemented with 10(-8) mmol L-1 17 beta-estradiol using embryo explant. The highest percentage of shoot-forming explants (100%) generally was obtained from embryo and plumule cultured in the MS culture medium with low MSH concentration. In addition, the highest CIR (100%) was obtained from embryo and plumule explant cultured in MS medium containing 10(-10) mmol L-1 of all MSH types. In conclusion, we observed that mammalian sex hormones may be used in bean in vitro culture.en_US
dc.description.sponsorshipUniversity of Helsinki; Helsinki University Libraryen_US
dc.description.sponsorshipOpen access funding provided by University of Helsinki. We thank the European Mocular Biology Conference (EMBC) for bringing together dedicated researchers from two of its member states, Tuerkiye and Finland. We believe that our joint work is the frst step towards establishing research exchanges between our laboratories. We also thank the Helsinki University Library for supporting open access publication of our work.en_US
dc.identifier.doi10.1038/s41598-023-30090-4
dc.identifier.issn2045-2322
dc.identifier.issue1en_US
dc.identifier.pmid36849811en_US
dc.identifier.scopus2-s2.0-85149270066en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-023-30090-4
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12772
dc.identifier.volume13en_US
dc.identifier.wosWOS:000986236800022en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNature Portfolioen_US
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleEffects of mammalian sex hormones on in vitro organogenesis of common bean (Phaseolus vulgaris L.)en_US
dc.typeArticleen_US

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