Cellular uptake and apoptotic potential of rhenium labeled magnetic protein cages in MDA-MB-231 cells

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dc.contributor.authorAsik, Elif
dc.contributor.authorAslan, Tugba Nur
dc.contributor.authorGuray, N. Tulin
dc.contributor.authorVolkan, Murvet
dc.date.accessioned2024-02-23T14:02:53Z
dc.date.available2024-02-23T14:02:53Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractRe-188-magnetoferritin nanoparticles (NPs) provide an attractive platform for localized radiation therapy due to their magnetic targeting capability while enhancing contrast in magnetic resonans imaging (MRI) signals. In this study, cellular uptake, in vitro cytotoxicity, apoptotic potential of a non-radioactive isotope of rhenium in the form of Re-187-magnetoferritin NPs were evaluated in both human normal mammary epithelial and breast metastatic adenocarcinoma cell lines. The results showed that, NP administration into the cells is through receptor mediated endocytosis and cancer cells displayed significantly higher uptake and cytotoxicity compared to normal cells. IC50 values of nanoparticles were calculated as 0.96 mg/mL for cancer and 1.73 mg/mL for normal cells. Annexin V/ Propidium Iodide (PI) staining also showed that, NPs induced higher apoptotic rates in cancer cells compared to normal cells. Gene expression analyses confirming the results showed that, pro-apoptotic PUMA and BAX genes were significantly up-regulated while anti-apoptotic BCL-2 and SURVIVIN genes were down-regulated in cancer cells compared to normal cells. Overall, these in vitro results suggest that, Re-187-magnetoferritin NPs have a promising potential for cancer therapy and can be used for imaging and diagnostic purposes for breast cancer at concentrations lower than 0.96 mg/mL. At concentrations above 1 mg/mL, NPs induce apoptosis which can also be used for cancer treatments.en_US
dc.description.sponsorshipResearch Fund of METU OYP Grant [BAP-08-11-DPT-2011K121010]en_US
dc.description.sponsorshipThis study was funded by the Research Fund of METU OYP Grant No: BAP-08-11-DPT-2011K121010.en_US
dc.identifier.doi10.1016/j.etap.2018.08.014
dc.identifier.endpage134en_US
dc.identifier.issn1382-6689
dc.identifier.issn1872-7077
dc.identifier.pmid30223109en_US
dc.identifier.scopus2-s2.0-85053409823en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1016/j.etap.2018.08.014
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11876
dc.identifier.volume63en_US
dc.identifier.wosWOS:000447481300017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEnvironmental Toxicology And Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetoferritinen_US
dc.subjectCytotoxicityen_US
dc.subjectApoptosisen_US
dc.subjectBreast Cancer Cell Linesen_US
dc.titleCellular uptake and apoptotic potential of rhenium labeled magnetic protein cages in MDA-MB-231 cellsen_US
dc.typeArticleen_US

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