Effects of Novel Root Repair Materials on Attachment and Morphological Behaviour of Periodontal Ligament Fibroblasts: Scanning Electron Microscopy Observation

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dc.contributor.authorAkbulut, Makbule Bilge
dc.contributor.authorArpaci, Pembegul Uyar
dc.contributor.authorEldeniz, Ayce Unverdi
dc.date.accessioned2024-02-23T13:03:28Z
dc.date.available2024-02-23T13:03:28Z
dc.date.issued2016
dc.departmentNEÜen_US
dc.description.abstractThe aim of this study was to evaluate the adhesion of periodontal ligament fibroblasts (PDLs) on newly proposed root repair materials [Biodentine, MM-MTA, polymethylmethacrylate (PMMA) bone cement, and SDR], in comparison with contemporary root repair materials [IRM, Dyract compomer, ProRoot MTA (PMTA), and Vitrebond]. Five discs from each material were fabricated in sterile Teflon molds, and the specimens were aged and prewetted in cell culture media for 96 hours. Three material discs were used for scanning electron microscopy (SEM) for the assessment of the attachment, density, and morphological changes in the PDLs, while two samples were used for energy dispersive x-ray spectroscopy (SEM-EDX) to determine the elemental composition of the materials. Human PDLs were plated onto the materials at a density of 10,000/well, and incubated for 3 days. The SEM micrographs were taken at different magnifications (5003 and 50003). In the SEM, the cells were attached and well spread-out on the surfaces of the Biodentine, PMTA, and Dyract compomer, while varied cell densities and morphological alterations were observed in the Vitrebond, IRM, MM-MTA, SDR, and PMMA bone cement groups. The SEM-EDX analysis revealed a maximum calcium percentage in the PMTA specimens, as well a maximum silicon percentage in the Dyract compomer specimens. This in vitro study demonstrated that the Biodentine and Dyract compomer supported PDL cell adhesion and spreading. The PMTA presented a favorable scaffold for better attachment of the PDL cell aggregates. Therefore, the calcium and silicon content of a material may enhance the PDL cell attachment.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordination [12202016]en_US
dc.description.sponsorshipThis study was supported by a grant given by Selcuk University Scientific Research Projects Coordination (No:12202016)en_US
dc.identifier.doi10.1002/jemt.22780
dc.identifier.endpage1221en_US
dc.identifier.issn1059-910X
dc.identifier.issn1097-0029
dc.identifier.issue12en_US
dc.identifier.pmid27647819en_US
dc.identifier.scopus2-s2.0-84988358557en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1214en_US
dc.identifier.urihttps://doi.org/10.1002/jemt.22780
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10622
dc.identifier.volume79en_US
dc.identifier.wosWOS:000388719900012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofMicroscopy Research And Techniqueen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell Attachmenten_US
dc.subjectPerforation Repair Materialsen_US
dc.subjectPeriodontal Ligament Fibroblastsen_US
dc.subjectSemen_US
dc.subjectSemen_US
dc.subjectEdxen_US
dc.titleEffects of Novel Root Repair Materials on Attachment and Morphological Behaviour of Periodontal Ligament Fibroblasts: Scanning Electron Microscopy Observationen_US
dc.typeArticleen_US

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