What is the protective effect of preischemic kisspeptin-10 administration against ischemia/reperfusion injury of striatum on mice?

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dc.contributor.authorAkkaya, Hatice
dc.contributor.authorSumer, Engin
dc.contributor.authorKutlu, Selim
dc.contributor.authorSolak, Hatice
dc.contributor.authorYilmaz, Bayram
dc.date.accessioned2024-02-23T14:41:44Z
dc.date.available2024-02-23T14:41:44Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractBackground/aim: Kisspeptin is a neuropeptide with a primary role on the onset of puberty and has beneficial effects on ischemia/reperfusion (I/R) injury. In this study, we aimed to investigate the effect of kisspeptin administration on striatal I/R injury in mice. Material and methods: Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups. The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation. A microdialysis probe was placed in the right striatum according to stereotaxic coordinates. During the experimental period, artificial cerebrospinal fluid was passed through the micropump. Then, transient cerebral ischemia was established by compressing both common carotid arteries with an aneurysm clip for 15 min and animals were reperfused for 2 h. Throughout the process of microdialysis (before, during and after I/R period), samples were collected to measure dopamine (DA), noradrenaline (NA), and 3,4-dihydroxyphenylglycine (DHPG) at intervals of 20 min continuously. At the end of the reperfusion period, the animals were decapitated, striatum was dissected, half of the animals were used for oxidative stress analyses (reduced glutathione, glutathione S-transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), and the other half were used for histopathology analyses. Results: Number of glial cells was significantly increased in kisspeptin-administered groups. DA levels in ischemic animals were decreased by kisspeptin administration (p < 0.0001). NA levels were reduced in animals administered with kisspeptin without I/R injury (p < 0.05). DHPG levels reduced during the reperfusion period in ischemic animals (p < 0.05). Kisspeptin did not exhibit a significant antioxidant activity in the ischemic animals, while GST and SOD levels were reduced in the I/R + kisspeptin group compared to the kisspeptin group (p < 0.05). Conclusion: Our results suggest that kisspeptin may be regulating the neurotransmitter release and metabolism, as well as inflammatory response in brain upon I/R injury.en_US
dc.identifier.doi10.55730/1300-0144.5493
dc.identifier.endpage1542en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.pmid36422497en_US
dc.identifier.scopus2-s2.0-85140629509en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1532en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5493
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16977
dc.identifier.volume52en_US
dc.identifier.wosWOS:000890907200013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCerebral Ischemia/Reperfusion Injuryen_US
dc.subjectKisspeptinen_US
dc.subjectNeurotransmitteren_US
dc.subjectMouseen_US
dc.subjectOxidative Stressen_US
dc.titleWhat is the protective effect of preischemic kisspeptin-10 administration against ischemia/reperfusion injury of striatum on mice?en_US
dc.typeArticleen_US

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