Investigation of Bacterial Gastroenteritis Agents in Patients Who Admitted to Pediatric Clinic with Diarrhea

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dc.contributor.authorAgrali, Muhammet Sukru
dc.contributor.authorDogan, Metin
dc.date.accessioned2024-02-23T14:35:23Z
dc.date.available2024-02-23T14:35:23Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractObjective: In this study we aimed to determine bacterial causes of diarrhea in patients presenting to the pediatric clinic. Methods: The stool specimens of 487 patients with diarrhea from pediatric emergency and other pediatric clinics were investigated in order to determine gastroenteritis causes. Microscopic examinations, and appropriate culture methods were performed to determine Salmonella, Shigella, E. coil O157: H7, Campylobacter, Yersinia enterocolitica, Vibrio cholerae, and Aeromonas species. Stool specimens of patients with diarrhea were stained with methylene blue for microscopic examination, and they were cultured on propagating selenite F medium for Salmonella spp. and Shigellas spp., and 8 h later, were subcultured to Salmonella Shigella agar. Also they were cultured to hektoen and xylose lysine desoxycholate agar, to Sorbitol MacConkey agar for E. cob O157: H7 strain, to MacConkey agar for Yersinia enterocolitica, to Campylobacter selective agar for Campylobacter spp., to vibrio selective agar for Vibrio cholerae and to 5% sheep blood agar for Aeromonas spp.. The isolated microorganisms were identified by conventional methods, biochemical tests, specific antibody/antigens and VITEK* 2 (bioMerieux, Marcy l'Etoile, France) device. Results: Salmonella spp. was the most frequently isolated bacteria that was isolated in 19 (3.9%) samples, and Campylobacter spp. was isolated in seven (1.4%), and E. coil O157:H7 and Aeromonas spp. were isolated in three (0.6%) samples. We observed that Salmonella isolation was highest in HE agar. It is remarkable that E. coil O157: H7 was observed among the bacterial agents of gastroenteritis in children. Conclusions: We concluded that E. coil O157: 117 strain should be investigated especially in stool samples with bloody diarrhea in our region, and bacteria such as Campylobacter spp. Aeromonas spp. should be investigated in addition to Salmonella spp. for investigation of gastroenteritis agents in children.en_US
dc.identifier.doi10.36519/kd.2021.3683
dc.identifier.endpage224en_US
dc.identifier.issn1301-143X
dc.identifier.issn1309-1484
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85126547645en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage219en_US
dc.identifier.urihttps://doi.org/10.36519/kd.2021.3683
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16004
dc.identifier.volume34en_US
dc.identifier.wosWOS:000756838600009en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherDoc Design Informatics Co Ltden_US
dc.relation.ispartofKlimik Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChilden_US
dc.subjectE. Coli O157: 117en_US
dc.subjectGastroenteritisen_US
dc.subjectDiarrheaen_US
dc.subjectSalmonellaen_US
dc.titleInvestigation of Bacterial Gastroenteritis Agents in Patients Who Admitted to Pediatric Clinic with Diarrheaen_US
dc.typeArticleen_US

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