Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

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dc.contributor.authorDuman, Selcuk
dc.contributor.authorAktan, Tahsin Murad
dc.contributor.authorCuce, Gokhan
dc.contributor.authorCihantimur, Bulent
dc.contributor.authorTokac, Mehmet
dc.contributor.authorAkbulut, Habip
dc.date.accessioned2024-02-23T14:37:39Z
dc.date.available2024-02-23T14:37:39Z
dc.date.issued2013
dc.departmentNEÜen_US
dc.description.abstractThe aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental step in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C-showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit (R) machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.en_US
dc.identifier.doi10.4067/S0717-95022013000100009
dc.identifier.endpage69en_US
dc.identifier.issn0717-9502
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84878763719en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage64en_US
dc.identifier.urihttps://doi.org/10.4067/S0717-95022013000100009
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16182
dc.identifier.volume31en_US
dc.identifier.wosWOS:000321868400009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Chilena Anatomiaen_US
dc.relation.ispartofInternational Journal Of Morphologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdipose Tissueen_US
dc.subjectStem Cellen_US
dc.subjectAngiogenesisen_US
dc.titleEffects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissueen_US
dc.typeArticleen_US

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