In vitro assessment of Momordica charantia/Hypericum perforatum oils loaded PCL/Collagen fibers: Novel scaffold for tissue engineering

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dc.contributor.authorEdiz, Emre Fatih
dc.contributor.authorGunes, Cansu
dc.contributor.authorKars, Meltem Demirel
dc.contributor.authorAvci, Ahmet
dc.date.accessioned2024-02-23T14:27:11Z
dc.date.available2024-02-23T14:27:11Z
dc.date.issued2024
dc.departmentNEÜen_US
dc.description.abstractThe research on tissue engineering applications has been progressing to manufacture ideal tissue scaffold biomaterials. In this study, a double-layered electrospun biofiber scaffold biomaterial including Polycaprolactone (PCL)/Collagen (COL) fibrous inner layer and PCL/ Momordica charantia (MC) and Hypericum perforatum (HP) oils fibrous outer layer was developed to manufacture a functional, novel tissue scaffold with the advantageous mechanical and biological properties. The main approach was to combine the natural perspective using medicinal oils with an engineering point of view to fabricate a potential functional scaffold for tissue engineering. Medicinal plants MC and HP are rich in functional oils and incorporation of them in a tissue scaffold will unveil their potential to augment both new tissue formation and wound healing. In this study, a novel double-layered scaffold prototype was fabricated using electrospinning technique with two PCL fiber layers, first is composed of collagen, and second is composed of oils extracted from medicinal plants. Initially, the composition of plant oils was analyzed. Thereafter the biofiber scaffold layers were fabricated and were evaluated in terms of morphology, physicochemistry, thermal and mechanical features, wettability, in vitro bio-degradability. Double-layered scaffold prototype was further analyzed in terms of in vitro biocompatibility and antibacterial effect. The medicinal oils blend provided antioxidant and antibacterial properties to the novel PCL/Oils layer. The results signify that inner PCL/COL layer exhibited advanced biodegradability of 8.5% compared to PCL and enhanced wettability with 11.7(degrees) contact angle. Strength of scaffold prototype was 5.98 N/mm(2) thanks to the elastic PCL fibrous matrix. The double-layered functional biofiber scaffold enabled 92% viability after 72 h contact with fibroblast cells and furthermore provided feasible attachment sites for the cells. The functional scaffold prototype's noteworthy mechanical, chemical, and biological features enable it to be suggested as a different novel biomaterial with the potential to be utilized in tissue engineering applications.en_US
dc.description.sponsorshipHorizon Europe project REGENEU [101079123]; TUBITAK 2244 University Industry Collaboration Project [119C100]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the Horizon Europe project REGENEU with grant number 101079123. TUBITAK 2244 University Industry Collaboration Project (119C100) is also acknowledged for the PhD scholarship to the first authoren_US
dc.identifier.doi10.1177/22808000231221067
dc.identifier.issn2280-8000
dc.identifier.pmid38217369en_US
dc.identifier.scopus2-s2.0-85182145480en_US
dc.identifier.urihttps://doi.org/10.1177/22808000231221067
dc.identifier.urihttps://hdl.handle.net/20.500.12452/14475
dc.identifier.volume22en_US
dc.identifier.wosWOS:001142808800001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofJournal Of Applied Biomaterials & Functional Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectComposite Biofiberen_US
dc.subjectCollagenen_US
dc.subjectHypericum Perforatumen_US
dc.subjectMomordica Charantiaen_US
dc.subjectScaffolden_US
dc.subjectTissue Engineeringen_US
dc.titleIn vitro assessment of Momordica charantia/Hypericum perforatum oils loaded PCL/Collagen fibers: Novel scaffold for tissue engineeringen_US
dc.typeArticleen_US

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