Lab-in-a-syringe using gold nanoparticles for rapid colorimetric chiral discrimination of enantiomers

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dc.contributor.authorZor, Erhan
dc.contributor.authorBekar, Nisa
dc.date.accessioned2024-02-23T14:02:16Z
dc.date.available2024-02-23T14:02:16Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractNanomaterials with different characteristics are offering many ingenious sensing approaches with interest for simple and disposable paper-based (bio)sensing applications. In this study, the calorimetric discrimination of alanine enantiomers is examined and, more importantly, AuNPs-embedded paper-based lab-in-a-syringe (LIS) device is developed as a sensing strategy. The LIS consists of two cellulose acetate membranes: the conjugate pad capturing the analyte and the detection pad signaling the presence of the captured analyte, both are sandwiched between reusable plastic filter holders connected to a disposable syringe. The principle of LIS assay is based on the enantioselective interaction occurring between the inherently chiral AuNPs and enantiomers in the first filter holder, which results in aggregation of AuNPs to give a distinct colour change from red to purple in solution and finally the aggregated AuNPs is kept on the detection pad through vertical-flow operation. AuNPs show an enantioselective recognition response toward L-Alanine and limit of detection (LOD) value is determined as 0.77 mM. In addition, we demonstrate the efficiency of the LIS device for detecting L-Alanine in human serum. The proposed LIS assay has some advantages such as useful for naked-eye observation, disposable, not time-consuming, inexpensive, no need of advanced instruments, easy to prepare and easy to handle. In the field, the approach which is the first demonstration of applicability of LIS device to show simple calorimetric enantioselective sensing of chiral species with a fast readout in less than 5 min is truly new and may have broad interest in enantiosensing of various chiral molecules.en_US
dc.identifier.doi10.1016/j.bios.2016.12.031
dc.identifier.endpage216en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid28011416en_US
dc.identifier.scopus2-s2.0-85006789377en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage211en_US
dc.identifier.urihttps://doi.org/10.1016/j.bios.2016.12.031
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11647
dc.identifier.volume91en_US
dc.identifier.wosWOS:000395599000032en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGold Nanoparticlesen_US
dc.subjectPaper-Based Sensoren_US
dc.subjectLab-In-A-Syringe Deviceen_US
dc.subjectChiral Recognitionen_US
dc.subjectColorimetric Assayen_US
dc.subjectVisual Detectionen_US
dc.titleLab-in-a-syringe using gold nanoparticles for rapid colorimetric chiral discrimination of enantiomersen_US
dc.typeArticleen_US

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