Exploring genetic diversity and Population structure of five Aegilops species with inter-primer binding site (iPBS) markers

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dc.contributor.authorKizilgeci, Ferhat
dc.contributor.authorBayhan, Bora
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorYildirim, Mehmet
dc.date.accessioned2024-02-23T13:59:23Z
dc.date.available2024-02-23T13:59:23Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractBackground Turkey is not only a center of origin for wheat, but also contains wild forms of various cereals. Turkey, located in the Fertile Crescent, has conserved its genetic richness to the present day. The aim of the study was to investigate the genetic diversity of 70 wild wheat species, to evaluate the structure of diversity in germplasm and to generate useful data for further breeding programs. Methods and results Genetic diversity and population structure of 70 wild wheat species (Ae. cylindrica, Ae. geniculata, Ae. triuncialis, T. dicocoides, Ae. columnaris) collected from Eastern and Southeastern Anatolia regions of Turkey were investigated in this study with the use of inter-primer binding site (iPBS) markers. Of 35 iPBS primers used, 11 yielded a total of 61 alleles. Number of alleles per marker varied between 2 (iPBS-2085) and 9 (iPBS-2394) with an average value of 5.55. Polymorphic information content (PIC) values varied between 0.22 and 0.47, with an average value of 0.35. Average number of effective alleles (Ne) was identified as 1.9488, Nei's genetic diversity (H) as 0,4861 and Shannon's information index (I) as 0.6791. Cluster analysis through unweighted pair-group mean average (UPGMA) method revealed that 70 wild wheats were divided into three main clusters. Genetic similarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 19% (YB2 and YB70) and 98% (YB66 and YB67). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 62.33% of total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2666 (the first sub-population) and 0.2330 (third sub-population), with an average value of 0.2500. Average population differentiation measurement (Fst) was identified as 0.3716 for the first sub-population, 0.3930 for the second subpopulation and 0.4804 for the third sub-population. Conclusions Based on present findings population structure of 70 wild wheat genotypes collected from Eastern and Southeastern Anatolia regions of Turkey were successfully characterized with the use of iPBS markers. Present findings suggested that iPBS-retrotransposon markers could reliably be used to elucidate genetic diversity of Aegilops genotypes.en_US
dc.description.sponsorshipScientific Research Projects Department of Mardin Artuklu University [MAU.BAP.20]en_US
dc.description.sponsorshipThis research was financially supported by Scientific Research Projects Department of Mardin Artuklu University (Grant Number: MAU.BAP.20.KMY.024).en_US
dc.identifier.doi10.1007/s11033-022-07689-3
dc.identifier.endpage8574en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue9en_US
dc.identifier.pmid35842855en_US
dc.identifier.scopus2-s2.0-85134531644en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage8567en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07689-3
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11162
dc.identifier.volume49en_US
dc.identifier.wosWOS:000827715500002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic Diversityen_US
dc.subjectIpbs Markersen_US
dc.subjectPopulation Structureen_US
dc.subjectWild Wheaten_US
dc.titleExploring genetic diversity and Population structure of five Aegilops species with inter-primer binding site (iPBS) markersen_US
dc.typeArticleen_US

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