The Presence of OXA Type Carbapenemases in Pseudomonas Strains: First Report from Turkey

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dc.contributor.authorEsenkaya Tasbent, Fatma
dc.contributor.authorOzdemir, Mehmet
dc.date.accessioned2024-02-23T14:41:47Z
dc.date.available2024-02-23T14:41:47Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractPseudomonas spp. that are one of the important nosocomial pathogens worldwide, and carbapenem resistance is observed in an increasing rate. Major factors leading to carbapenem resistance are metallo-beta-lactamases (MBLs) and carbapenem-hydrolyzing class D oxacillinases. MBLs are frequently prevalent in Pseudomonas spp., while carbapenem-hydrolyzing class D oxacillinases has been almost exclusively found in multidrug and carbapenem-resistant Acinetobacter baumannii. The aim of this study was to investigate the presence of OXA-23, OXA-40 and OXA-58 genes that encode carbapenemases, in carbapenem-resistant Pseudomonas spp. strains. A total of 184 imipenem and/or meropenem-resistant Pseudomonas spp. strains isolated from different clinical samples (85 bronchoalveolar lavage, 31 wound, 18 tracheal aspirate, 16 urine, 14 blood, 10 sputum, 3 catheter, 3 throat, 2 drainage fluid, 1 abscess, 1 peritoneal fluid) in Medical Microbiology Laboratory of Necmettin Erbakan University Meram Faculty of Medicine, between November 2011 to October 2013, were included in the study. The isolates were identified by conventional methods and an automated system (VITEK-2 Compact, bioMerieux, France), while the antibiotic susceptibility tests were performed with Kirby-Bauer disk diffusion method and automated system according to the recommendations of CLSI. The presence of OXA-23, OXA-40 and OXA-58 genes in strains were investigated by a commercial PCR kit (Hyplex CarbOxa ID; Amp lex Diagnostics GmbH, Germany). Molecular studies were carried out in three steps, namely extraction of genomic DNA; multiplex PCR amplification and hybridization. In the final step, hybridization was achieved in the ELISA-based system. In our study, 12 (6.5%) out of 184 carbapenem-resistant Pseudomonas spp. strains were positive for OXA-23, 1 (0.54%) for OXA-40 and 1 (0.54%) for OXA-58, with a total positive rate of 7.6% (n= 14). Most of the carbapenem-resistant Pseudomonas spp. strains (129/184; 70%) were isolated from the samples of patients in intensive-care units, and bronchoalveolar lavage specimens were the most prevalent samples (85/184; 46.2%). This study determined the presence and frequency of OXA type carbapenemases among Pseudomonas strains for the first time in our region and Turkey. These resistance genes demonstrated in Acinetobacter spp. were also detected in this study in Pseudomonas spp. and they may cause carbapenem resistance directly or by affecting the other resistance mechanisms with a synergistic effect. This study is expected to provide a basis for further studies to elucidate resistance mechanisms.en_US
dc.identifier.doi10.5578/mb.8563
dc.identifier.endpage34en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid25706728en_US
dc.identifier.scopus2-s2.0-84924808321en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.5578/mb.8563
dc.identifier.urihttps://hdl.handle.net/20.500.12452/17007
dc.identifier.volume49en_US
dc.identifier.wosWOS:000350946600003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPseudomonasen_US
dc.subjectOxa Genesen_US
dc.subjectCarbapenemaseen_US
dc.subjectCarbapenem Resistanceen_US
dc.titleThe Presence of OXA Type Carbapenemases in Pseudomonas Strains: First Report from Turkeyen_US
dc.typeArticleen_US

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