Tomentosin induces apoptosis in pancreatic cancer cells through increasing reactive oxygen species and decreasing mitochondrial membrane potential

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dc.contributor.authorGueclue, Ebru
dc.contributor.authorAyan, Ilknur Cinar
dc.contributor.authorDursun, Hatice Guel
dc.contributor.authorVural, Hasibe
dc.date.accessioned2024-02-23T14:16:22Z
dc.date.available2024-02-23T14:16:22Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractThe aim of this study was to determine possible anticancer effect of tomentosin, a natural sesquiterpene lactone, on pancreatic cancer cells. The cytotoxic effect of tomentosin was determined by XTT analysis. Colony formation and apoptosis analyzes were performed, Reactive oxygen species (ROS) level and change in mitochondrial membrane potential (MMP) were evaluated in control and tomentosin-treated cells. The effect of tomentosin on expression levels of apoptosis-related genes was determined by qRT-PCR and Caspase-3 and Caspase-9 proteins were analyzed by western blot. And, the effect of tomentosin on migration and invasion of cells were evaluated. The IC50 dose of tomentosin was found to be 31.11 mu M in PANC-1 cells and 33.93 mu M in MIA PaCa-2 cells for 48 h. And, treatment of tomentosin at IC50 dose suppressed the colony forming capacity of cells. While tomentosin increased apoptosis rate and ROS production, an decrease was observed in MMP. Tomentosin affected expression level of apoptosis-related genes and increased Caspase-3 and Caspase-9 protein levels. After tomentosin treat-ment, cell migration and invasion were suppressed. As a result, this study reveals that tomentosin has anticancer effects on pancreatic cancer cells, and therefore it predicts that tomentosin can be evaluated as an effective agent against pancreatic cancer.en_US
dc.identifier.doi10.1016/j.tiv.2022.105458
dc.identifier.issn0887-2333
dc.identifier.issn1879-3177
dc.identifier.pmid35988885en_US
dc.identifier.scopus2-s2.0-85136488037en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.tiv.2022.105458
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12642
dc.identifier.volume84en_US
dc.identifier.wosWOS:000848387200004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxicology In Vitroen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTomentosinen_US
dc.subjectPancreatic Canceren_US
dc.subjectApoptosisen_US
dc.subjectMigrationen_US
dc.subjectInvasionen_US
dc.titleTomentosin induces apoptosis in pancreatic cancer cells through increasing reactive oxygen species and decreasing mitochondrial membrane potentialen_US
dc.typeArticleen_US

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