Effects of mammalian sex hormones on regeneration capacity, retrotransposon polymorphism and genomic instability in wheat (Triticum aestivum L.)

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dc.contributor.authorTurkoglu, Aras
dc.date.accessioned2024-02-23T13:59:26Z
dc.date.available2024-02-23T13:59:26Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractMammalian steroid hormones (MSH) are also inherently synthesized by several plant species. However, external application of steroid hormones further stimulates cell division, pollen germination, plant growth and development. There is little known about the effects of MSH on polymorphism and DNA damage in Triticum aestivum L under in vitro conditions. In this study, inter-primer binding site (iPBS) retrotransposon and coupled restriction enzyme digestion-iPBS (CRED-iPBS) markers were used for detection of the variation in responded embryogenic callus (REC) that were obtained from endosperm-supported mature embryo of wheat in Murashige and Skoog (MS) medium containing different concentrations [0 (control), 10(-4), 10(-6) and 10(-8) m mol L-1] of MSH (progesterone, 17 beta-estradiol, estrone and testosterone). Responded embryogenic callus (REC) and regenerable callus (RE) induction from mature embryos varied with MSH type and concentrations. The highest level of genomic template stability (GTS) value (80.52%) was obtained from 10(-8) mM progesterone treatments to the lowest value (68.83%) from 10(-4) mM 17 beta-estradiol treatments. Epigenetic changes were more frequent and variable than the genetic changes. While DNA hypermethylation was observed at higher 17 beta-estradiol concentrations, DNA hypomethylation was observed in progesterone treatments. It was concluded based on iPBS and CRED-iPBS findings that high MSH concentrations caused DNA changes and methylation. Key message Variability among regenerates caused by MSH treatments in different concentration were observed. iPBS polymorphisms indicated the presence of genetic variation among the wheat regenerates. Application of MSH in high concentration had a clearly effective on polymorphism and genomic instabilityin the wheat mature embryo.en_US
dc.identifier.doi10.1007/s11240-022-02440-z
dc.identifier.endpage659en_US
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85138906820en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage647en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-022-02440-z
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11193
dc.identifier.volume152en_US
dc.identifier.wosWOS:000905894700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenomic Template Stabilityen_US
dc.subjectPolyaminesen_US
dc.subjectIpbsen_US
dc.subjectMshen_US
dc.titleEffects of mammalian sex hormones on regeneration capacity, retrotransposon polymorphism and genomic instability in wheat (Triticum aestivum L.)en_US
dc.typeArticleen_US

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