Sodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques

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dc.contributor.authorTurkoglu, Aras
dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorTosun, Metin
dc.contributor.authorSzulc, Piotr
dc.contributor.authorDemirel, Fatih
dc.contributor.authorEren, Baris
dc.contributor.authorBujak, Henryk
dc.date.accessioned2024-02-23T14:35:05Z
dc.date.available2024-02-23T14:35:05Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractWheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a variety that may be used in selective breeding programs as well as functional gene investigations. The present experiment was used to evaluate the potential application of a conventional chemical mutagenesis technique via sodium azide (NaN3) for the germination and seedling growth stage in wheat. Experiments with NaN3 mutagenesis were conducted using four different treatment periods (0, 1, 2, and 3 h) and five different concentrations (0, 0.5, 1, 1.5, and 2 mM). The genomic instability and cytosine methylation of wheat using its seeds were investigated after they were treated. In order to evaluate the genomic instability and cytosine methylation in wheat that had been treated, interprimer binding site (iPBS) markers were used. The mutagenic effects of NaN3 treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat plants. The results of the experiment showed considerable changes in the iPBS profiles produced by the administration of the same treatments at different dosages and at different times. Coupled restriction enzyme digestion interprimer binding site (CRED-iPBS) assays identified changes in gDNA cytosine methylation. The highest polymorphism value was obtained during 1 h + 2 mM NaN3, while the lowest (20.7%) was obtained during 1 h + 1.5 mM NaN3. Results showed that treatments with NaN3 had an effect on the level of cytosine methylation and the stability of the genomic template in wheat plants in the germination stage. Additionally, an integrated method can be used to for mutation-assisted breeding using a molecular marker system in wheat followed by the selection of desired mutants.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TOVAG 113O940]en_US
dc.description.sponsorshipThis research received no external funding.en_US
dc.identifier.doi10.3390/agriculture13061242
dc.identifier.issn2077-0472
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85164195996en_US
dc.identifier.urihttps://doi.org/10.3390/agriculture13061242
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15850
dc.identifier.volume13en_US
dc.identifier.wosWOS:001013898500001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofAgriculture-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenomic Template Stabilityen_US
dc.subjectDna Methylationen_US
dc.subjectMutagenen_US
dc.subjectIpbsen_US
dc.titleSodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniquesen_US
dc.typeArticleen_US

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