The effect of medicarpin on PTEN/AKT signal pathway in head and neck squamous cell carcinoma

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dc.contributor.authorYigin, Aysel Kalayci
dc.contributor.authorDonmez, Huseyin
dc.contributor.authorHitit, Mustafa
dc.contributor.authorSeven, Sena
dc.contributor.authorEser, Nadire
dc.contributor.authorKurar, Ercan
dc.contributor.authorSeven, Mehmet
dc.date.accessioned2024-02-23T14:37:53Z
dc.date.available2024-02-23T14:37:53Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractBackground/Aim: We aimed to investigate the in vitro modulating effects of medicarpin on the PI3K/AKT signal pathway gene expressions in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: The effect of medicarpin on PTEN and other associated genes in the PTEN/AKT signal pathway was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, real-time quantitative polymerase chain reaction, and Western blot analysis in the SCCL-MT1 (HNSCC) and control (HEK-293) cell lines. Results: The IC50 dose was 80 mu M as a result of medicarpin treatment on HNSCC cells (P = 0.0006). It was found that PTEN and AKT gene expressions increased after the medicarpin administration while PDK1 gene expression was decreased in SCCL-MT1 cells (P = 0.0002, P = 0.0003, and P = 0.05, respectively). Protein expression results showed that medicarpin-treated cells significantly increased in pAKT (P = 0.024), pPTEN (P = 0.032), and decreased pPDK1 (P = 0.059) in SCCL-MT1. Conclusions: Our data show that medicarpin modulates HNSCC cells by increasing the PTEN and decreasing PDK1 expressions. PDK1 gene expression effects mTOR pathway which may increase AKT gene. Our study suggests that both medicarpin extracts combination with the HNSCC drug may be more effective in cancer treatment. Future prospective studies that integrate molecular and pharmacogenetic studies are crucial for revealing the mechanism and preventive medical efforts.en_US
dc.identifier.doi10.4103/jcrt.jcrt_641_21
dc.identifier.endpage184en_US
dc.identifier.issn0973-1482
dc.identifier.issn1998-4138
dc.identifier.issue1en_US
dc.identifier.pmid35381781en_US
dc.identifier.scopus2-s2.0-85127711075en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage180en_US
dc.identifier.urihttps://doi.org/10.4103/jcrt.jcrt_641_21
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16256
dc.identifier.volume18en_US
dc.identifier.wosWOS:000788081000027en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofJournal Of Cancer Research And Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHead And Neck Squamous Cell Carcinomaen_US
dc.subjectIn Vitroen_US
dc.subjectMedicarpinen_US
dc.subjectPten/Akt Signal Pathwayen_US
dc.titleThe effect of medicarpin on PTEN/AKT signal pathway in head and neck squamous cell carcinomaen_US
dc.typeArticleen_US

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