Laboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodies

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dc.contributor.authorFeyzioglu, Bahadir
dc.contributor.authorYavru, Sibel
dc.contributor.authorOzdemir, Mehmet
dc.date.accessioned2024-02-23T14:16:52Z
dc.date.available2024-02-23T14:16:52Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractIntroduction Central nervous system (CNS) viral infections are a serious problem requiring accurate diagnosis and treatment. Human herpesviruses (HHVs) are an important cause of these infections. Recent research has focused on new diagnostic methods allowing accurate and rapid identification of viral infections because there are still diagnostic difficulties for these infections. This study was done to determine the etiologic role of human herpes viruses and to obtain information that will contribute to the diagnostic algorithm in suspected cases of viral encephalitis or aseptic meningitis. Materials and Methods In our study, herpes simplex virus (HSV)-1, HSV-2, varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and HHV-6 DNA was detected in the cerebrospinal fluid (CSF) by multiplex polymerase chain reaction (PCR) and virus-specific immunoglobulin G (IgG) antibodies in CSF and serum by EIA in pediatric encephalitis/meningitis cases. Results HSV-1 and VZV were detected in 5 and 3.3% of aseptic meningitis cases, respectively, but no encephalitis cases. Other viruses were not identified as etiologic agents. The seroprevalences were determined as 72.4, 34.3, 81.9, 93.3, 88.6, and 80.9%, respectively for HSV-1, HSV-2, VZV, CMV, EBV, and HHV-6. The performance of specific IgG CSF/serum antibody index (AI) was not satisfactory. Conclusion Our study indicates that the multiplex PCR method is the most suitable for the diagnosis of CNS infections caused by HHVs. However, due to the high cost of the PCR method, the positive results of the specific AI may be significant, but the negative results are unreliable, especially in limited health care facilities.en_US
dc.description.sponsorshipSelcuk University [11102036]en_US
dc.description.sponsorshipWe would like to thank the coordinator of the Scientific Research Projects of the Selcuk University for the support of our project (No: 11102036).en_US
dc.identifier.doi10.1055/s-0038-1639616
dc.identifier.endpage184en_US
dc.identifier.issn1305-7707
dc.identifier.issn1305-7693
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85044583352en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage178en_US
dc.identifier.urihttps://doi.org/10.1055/s-0038-1639616
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12842
dc.identifier.volume13en_US
dc.identifier.wosWOS:000441063800002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofJournal Of Pediatric Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibody Indexen_US
dc.subjectHuman Herpes Virusesen_US
dc.subjectMultiplex Pcren_US
dc.titleLaboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodiesen_US
dc.typeArticleen_US

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