Microcryogels as plastic antibodies for transferrin purification

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dc.contributor.authorCetin, Kemal
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-02-23T14:13:37Z
dc.date.available2024-02-23T14:13:37Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractMolecular imprinting technology (MIT) presents self-organization of functional monomers around a template, leading formation of specific recognition sites for the template with high selectivity. In this study, cryogels in microscale were fabricated as novel plastic antibodies via MIT for transferrin purification. After chemical and physical characterization studies, optimal hsTf adsorption conditions for the plastic antibodies were then determined by investigating parameters including medium pH and temperature, initial analyte concentration and salt concentration. Selectivity studies were performed by considering both size and isoelectric points of the proteins. Compared with the non-imprinted P(HEMATrp) microcryogels, the relative selectivity coefficients of the plastic antibodies for hsTf/MYB and hsTf/HSA were found to be 2.24 and 2.10 times higher, respectively. The findings indicated that the molecularly imprinted microcryogels could be selective, reusable and cost-friendly potential plastic antibodies for purification of transferrin.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)en_US
dc.description.sponsorshipKemal cetin thanks The Scientific and Technological Research Council of Turkey (TUBITAK) for the support by TUBITAK-BIDEB 2211-National Ph.D Scholarship Programme.en_US
dc.identifier.doi10.1016/j.procbio.2018.12.020
dc.identifier.endpage184en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85059750138en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage174en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2018.12.020
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12502
dc.identifier.volume79en_US
dc.identifier.wosWOS:000466621000022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrocryogelsen_US
dc.subjectMolecular Imprintingen_US
dc.subjectPlastic Antibodiesen_US
dc.subjectProtein Purificationen_US
dc.subjectTransferrinen_US
dc.titleMicrocryogels as plastic antibodies for transferrin purificationen_US
dc.typeArticleen_US

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