Determining Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces from Turkiye Using SSR Markers

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dc.contributor.authorOzkan, Guller
dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorOzturk, Halil Ibrahim
dc.contributor.authorElkoca, Erdal
dc.contributor.authorPoczai, Peter
dc.date.accessioned2024-02-23T14:35:09Z
dc.date.available2024-02-23T14:35:09Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractAssessment of genetic diversity among different varieties helps to improve desired characteristics of crops, including disease resistance, early maturity, high yield, and resistance to drought. Molecular markers are one of the most effective tools for discovering genetic diversity that can increase reproductive efficiency. Simple sequence repeats (SSRs), which are codominant markers, are preferred for the determination of genetic diversity because they are highly polymorphic, multi-allelic, highly reproducible, and have good genome coverage. This study aimed to determine the genetic diversity of 40 common bean (Phaseolus vulgaris L.) landraces collected from the Ispir district located in the Northeast Anatolia region of Turkiye and five commercial varieties using SSR markers. The Twenty-seven SSR markers produced a total of 142 polymorphic bands, ranging from 2 (GATS91 and PVTT001) to 12 (BM153) alleles per marker, with an average number of 5.26 alleles. The gene diversity per marker varied between 0.37 and 0.87 for BM053 and BM153 markers, respectively. When heterozygous individuals are calculated proportional to the population, the heterozygosity ranged from 0.00 to 1.00, with an average of 0.30. The expected heterozygosity of the SSR locus ranged from 0.37 (BM053) to 0.88 (BM153), with an average of 0.69. Nei's gene diversity scored an average of 0.69. The polymorphic information content (PIC) values of SSR markers varied from 0.33 (BM053) to 0.86 (BM153), with an average of 0.63 per locus. The greatest genetic distance (0.83) was between lines 49, 50, 53, and cultivar Karacasehir-90, while the shortest (0.08) was between lines 6 and 26. In cluster analysis using Nei's genetic distance, 45 common bean genotypes were divided into three groups and very little relationship was found between the genotypes and the geographical distances. In genetic structure analysis, three subgroups were formed, including local landraces and commercial varieties. The result confirmed that the rich diversity existing in Ispir bean landraces could be used as a genetic resource in designing breeding programs and may also contribute to Turkiye bean breeding programs.en_US
dc.description.sponsorshipHelsinki University Library; iASK Research Granten_US
dc.description.sponsorshipP.P. expresses his gratitude for the support of the iASK Research Grant. The authors thank the Helsinki University Library for supporting open-access publication.en_US
dc.identifier.doi10.3390/genes13081410
dc.identifier.issn2073-4425
dc.identifier.issue8en_US
dc.identifier.pmid36011321en_US
dc.identifier.scopus2-s2.0-85136709279en_US
dc.identifier.urihttps://doi.org/10.3390/genes13081410
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15908
dc.identifier.volume13en_US
dc.identifier.wosWOS:000846088500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofGenesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBean Breedingen_US
dc.subjectGenetic Diversityen_US
dc.subjectMolecular Markersen_US
dc.subjectStructureen_US
dc.titleDetermining Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces from Turkiye Using SSR Markersen_US
dc.typeArticleen_US

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