Plastid trnH-psbA intergenic spacer serves as a PCR-based marker to detect common grain adulterants of coffee (Coffea arabica L.)

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dc.contributor.authorUncu, Ali Tevfik
dc.contributor.authorUncu, Ayse Ozgur
dc.date.accessioned2024-02-23T14:02:57Z
dc.date.available2024-02-23T14:02:57Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description31st EFFoST International Conference - Food Science and Technology Challenges for the 21st Century - Research to Progress Society -- NOV 13-16, 2017 -- Sitges, SPAINen_US
dc.description.abstractThe present work describes the utilization of interspecific length polymorphisms within the chloroplastic trnH-psbA intergenic spacer to standardize a DNA-based method that identifies adulteration in Arabica coffee (Coffea arabica L.). Adulteration of coffee with roasted cereal grains and soybean is a common fraudulent practice, yet, research to design sensitive DNA-based tests in order to establish traceability in coffee is extremely limited. In the present study, PCR-CE (PCR-capillary electrophoresis) analysis of the trnH-psbA intergenic spacer proved successful to discriminate Arabica coffee and its common adulterants, namely corn, soybean, rice, wheat and barley. When the barcode PCR-CE assay was tested on admixtures, adulteration as low as 1% was successfully detected simply through barcode amplification and capillary electrophoresis separation. Moreover, it is important to note that the proposed barcode assay not only detects adulteration but also reveals the identity of the adulterant species based on barcode amplification profiles obtained from reference sets of anticipated adulterants. (C) 2018 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipEuropean Federat Food Sci & Technolen_US
dc.identifier.doi10.1016/j.foodcont.2018.03.029
dc.identifier.endpage39en_US
dc.identifier.issn0956-7135
dc.identifier.issn1873-7129
dc.identifier.scopus2-s2.0-85056340956en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage32en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodcont.2018.03.029
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11913
dc.identifier.volume91en_US
dc.identifier.wosWOS:000435057800006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Controlen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBarcodeen_US
dc.subjectChloroplast Genomeen_US
dc.subjectFood Authenticationen_US
dc.subjectGenotypingen_US
dc.subjectIndel Polymorphismen_US
dc.titlePlastid trnH-psbA intergenic spacer serves as a PCR-based marker to detect common grain adulterants of coffee (Coffea arabica L.)en_US
dc.typeConference Objecten_US

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