Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)

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dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorBalpinar, Ozge
dc.contributor.authorNadaroglu, Hayrunnisa
dc.contributor.authorAlayli, Azize
dc.contributor.authorPoczai, Peter
dc.date.accessioned2024-02-23T14:35:19Z
dc.date.available2024-02-23T14:35:19Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractNanomaterials with unique and diverse physico-chemical properties are used in plant science since they improve plant growth and development and offer protection against biotic and abiotic stressors. Previous studies have explored the effects of such nanomaterials on different plant mechanisms, but information about the effects of nanomaterials on induced DNA methylation, genomic instability and LTR retrotransposon polymorphism in wheat is lacking. Therefore, the present study highlights the key role of nanoparticles in DNA methylation and polymorphism in wheat by investigating the effects of ZnO, CuO and gamma-Fe3O4 nanoparticles (NPs) on mature embryo cultures of wheat (Triticum aestivum L.). Nanoparticles were supplemented with Murashige and Skoog (MS) basal medium at normal (1X), double (2X) and triple (3X) concentrations. The findings revealed different responses to the polymorphism rate depending on the nanoparticle type and concentration. Genomic template stability (GTS) values were used to compare the changes encountered in iPBS profiles. ZnO, CuO and gamma-Fe3O4 NPs increased the polymorphism rate and cytosine methylation compared to the positive control while reducing GTS values. Moreover, non-gamma-Fe3O4 NPs treatments and 2X ZnO and CuO NP treatments yielded higher polymorphism percentages in both MspI-and HpaII-digested CRED-iPBS assays and were thus classified as hypermethylation when the average polymorphism percentage for MspI digestion was considered. On the other hand, the 3X concentrations of all nanoparticles decreased HpaII and MspI polymorphism percentages and were thus classified as hypomethylation. The findings revealed that MS medium supplemented with nanoparticles had epigenetic and genotoxic effects.en_US
dc.description.sponsorshipiASK Research Granten_US
dc.description.sponsorshipPeter Poczai expresses his gratitude for the support of the iASK Research Grant. The authors thank the Helsinki University Library for supporting open access publication.en_US
dc.identifier.doi10.3390/plants11172193
dc.identifier.issn2223-7747
dc.identifier.issue17en_US
dc.identifier.pmid36079574en_US
dc.identifier.scopus2-s2.0-85137838697en_US
dc.identifier.urihttps://doi.org/10.3390/plants11172193
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15951
dc.identifier.volume11en_US
dc.identifier.wosWOS:000851823100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPlants-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDna Methylationen_US
dc.subjectGenomic Instabilityen_US
dc.subjectIn Vitroen_US
dc.subjectNanoparticlesen_US
dc.subjectRetrotransposonsen_US
dc.subjectWheaten_US
dc.titleEffects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)en_US
dc.typeArticleen_US

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