Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L-czeczottianus and L-nissolia

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dc.contributor.authorLlorent-Martinez, Eulogio J.
dc.contributor.authorZengin, Gokhan
dc.contributor.authorCordova, Maria L. Fernandez-De
dc.contributor.authorBender, Onur
dc.contributor.authorAtalay, Arzu
dc.contributor.authorCeylan, Ramazan
dc.contributor.authorMollica, Adriano
dc.date.accessioned2024-02-23T14:34:59Z
dc.date.available2024-02-23T14:34:59Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractMembers of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MSn, as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation). Cholinesterases (AChE and BChE), tyrosinase, alpha-amylase, and alpha-glucosidase were used to evaluate enzyme inhibitory effects. Moreover, vitexin (apigenin-8-C-glucoside) and 5-O-caffeoylquinic acid were further subjected to molecular docking experiments to provide insights about their interactions at molecular level with the tested enzymes. In vitro cytotoxic effects were examined against human embryonic kidney cells (HEK293) by using iCELLigence real time cell analysis system. Generally, L. czeczottianus exhibited stronger antioxidant properties than L. nissolia. However, L. nissolia had remarkable enzyme inhibitory effects against cholinesterase, amylase and glucosidase. HPLC-ESI-MSn analysis revealed that flavonoids were major components in these extracts. On the basis of these results, Lathyrus extracts were rich in biologically active components; thus, these species could be utilized to design new phytopharmaceutical and nutraceutical formulations.en_US
dc.description.sponsorshipUCLM Research Plan; Scientific and Technological Research Council of Turkey (TUBITAK) [113Z892]; UEFISCDI, Romania [PNII-RU-TE-2014-4-1247]en_US
dc.description.sponsorshipEJLM acknowledges the financial support from the UCLM Research Plan. CICT from University of Jaen is also acknowledged. This research was also supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project Number: 113Z892). AnM acknowledges funding from UEFISCDI, Romania, project no. PNII-RU-TE-2014-4-1247.en_US
dc.identifier.doi10.3389/fphar.2017.00083
dc.identifier.endpage20en_US
dc.identifier.issn1663-9812
dc.identifier.pmid28289386en_US
dc.identifier.scopus2-s2.0-85014192575en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.3389/fphar.2017.00083
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15843
dc.identifier.volume8en_US
dc.identifier.wosWOS:000394743900001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherFrontiers Media Saen_US
dc.relation.ispartofFrontiers In Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLathyrusen_US
dc.subjectFlavonoiden_US
dc.subjectAntioxidanten_US
dc.subjectCytotoxicen_US
dc.subjectMolecular Dockingen_US
dc.subjectBioactive Formulationsen_US
dc.titleTraditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L-czeczottianus and L-nissoliaen_US
dc.typeArticleen_US

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