The role of multiplex PCR test in identification of bacterial pathogens in lower respiratory tract infections

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dc.contributor.authorAydemir, Ozlem
dc.contributor.authorAydemir, Yusuf
dc.contributor.authorOzdemir, Mehmet
dc.date.accessioned2024-02-23T14:45:56Z
dc.date.available2024-02-23T14:45:56Z
dc.date.issued2014
dc.departmentNEÜen_US
dc.description.abstractObjectives: Lower respiratory tract infection is one of the most important causes of morbidity and mortality. However establishing a microbial diagnosis for patients with lower respiratory tract infection is still challenging and is often achieved in only half of cases by conventional methods. This study was designed to compare the fast responsive PCR method with the culture method in lower respiratory tract infections and to evaluate the reliability of multiplex PCR method. Methods: One hundred ninety seven patients with the symptoms of acute lower respiratory tract infection, and diagnosed with community-acquired pneumonia, acute exacerbation of chronic obstructive pulmonary disease and exacerbations of bronchiectasis were included in the study. Both culture and PCR methods was performed for the isolation of most commonly seen bacteria, from sputum, nasopharyngeal swabs and bronchoalveolar lavage fluid samples. Results: While at least one bacterial isolation was determined in 62 (31.5%) of all patients with culture method, this number increased to 125 (63.5%) with multiplex PCR. The bacteria most commonly identified by PCR were S. pneunnoniae (32%) and H. influenzae (31%). There was a significant difference between PCR and culture in terms of multi-factor detection rates (p < 0.005). Multiple bacteria were detected in only two cases in cultures; however, multiple pathogens were detected in 47 cases with PCR. Conclusions: Conventional methods, such as culture and serology are not always adequate to detect the pathogens in lower respiratory tract. Real-time PCR assays proved highly sensitive and rapid. The prevalence of bacteria and multiple agent detected by real-time PCR compared with culture was substantially higher. Widespread use of PCR methods, by providing the immediate and appropriate agent specific antibiotic treatment of LRTI, will help reduce failure and contributes to a reduction in antibiotic resistance.en_US
dc.identifier.endpage1016en_US
dc.identifier.issn1682-024X
dc.identifier.issue5en_US
dc.identifier.pmid25225517en_US
dc.identifier.scopus2-s2.0-84905118207en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1011en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/17701
dc.identifier.volume30en_US
dc.identifier.wosWOS:000342973000019en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherProfessional Medical Publicationsen_US
dc.relation.ispartofPakistan Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacterial Etiologyen_US
dc.subjectCultureen_US
dc.subjectLower Respiratory Tract Infectionen_US
dc.subjectPcren_US
dc.titleThe role of multiplex PCR test in identification of bacterial pathogens in lower respiratory tract infectionsen_US
dc.typeArticleen_US

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