Exploring the genetic diversity and population structure of fenugreek (Trigonella foenum-graecum L.) genotypes through inter-primer binding site (iPBS)-retrotransposon marker system

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dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorOzer, Hakan
dc.contributor.authorMelik, Seda
dc.contributor.authorCoban, Furkan
dc.contributor.authorTurkoglu, Aras
dc.date.accessioned2024-02-23T13:56:09Z
dc.date.available2024-02-23T13:56:09Z
dc.date.issued2024
dc.departmentNEÜen_US
dc.description.abstractFenugreek, a key medicinal-aromatic plant, offers rich bioactive compounds and nutritional value. Its diverse applications in cuisine and pharmaceuticals, coupled with health benefits like anti-diabetic and antioxidant properties, underscore its significance. Assessing genetic diversity becomes crucial for effective conservation and utilization. In this study, we examined the molecular diversity and population structure of 34 fenugreek genotypes collected from 18 countries worldwide using 24 inter-primer binding site (iPBS) markers. The iPBS primers produced 499 bands, with the total number of bands per primer ranging from 15 (iPBS-2224) to 26 (iPBS-2077), averaging 20.79. Polymorphism information content (PIC) ranged from 0.03 (iPBS-2374) to 0.34 (iPBS-2237), averaging 0.23. In the molecular analysis, the G1 genotype (Isfahan/Iran) exhibited the maximum effective number of alleles (Ne), Nie's gene diversity (He), and Shannon's information index (I) at 1.946, 0.486, and 0.679, respectively. Conversely, the G34 genotype (India/B) displayed the lowest values at 1.539, 0.350, and 0.535, respectively. Utilizing the unweighted pair-group means average (UPGMA) method, the iPBS-based tree revealed three distinct groups corresponding to the genomic constitution of fenugreek genotypes, a pattern partially corroborated by principal component analysis (PCA). Further model-based cluster analysis classified the 34 genotypes into four subpopulations, with expected heterozygosity (He) values of 0.428, 0.390, 0.426, and 0.007, respectively. The F-statistic (Fst) values for these subpopulations were 0.197, 0.210, 0.187, and 0.356, respectively. These findings underscored significant genetic variation among the tested fenugreek genotypes, thereby demonstrating the efficacy of iPBS markers in accurately assessing genetic diversity and phylogenetic relationships within fenugreek populations.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [220O003]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkiye (TUBITAK; Project No: 220O003).en_US
dc.identifier.doi10.1007/s10722-023-01849-5
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.scopus2-s2.0-85181968136en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s10722-023-01849-5
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11097
dc.identifier.wosWOS:001138473900001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofGenetic Resources And Crop Evolutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFenugreeken_US
dc.subjectPolymorphismen_US
dc.subjectPrincipal Component Analysisen_US
dc.subjectRetrotransposonsen_US
dc.titleExploring the genetic diversity and population structure of fenugreek (Trigonella foenum-graecum L.) genotypes through inter-primer binding site (iPBS)-retrotransposon marker systemen_US
dc.typeArticleen_US

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