Spectrophotometric quantification of paracetamol and tramadol hydrochloride by chemometric calibration methods

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dc.contributor.authorSelimoglu, Faysal
dc.contributor.authorPinarcik, Nermin
dc.date.accessioned2024-02-23T14:41:45Z
dc.date.available2024-02-23T14:41:45Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractThe results of UV spectrophotometric analysis were analysed using partial least squares (PLS) and principal component re-gression (PCR) techniques to allow simultaneous evaluation of tramadol hydrochloride (TRA) and p-acetaminophen (PAR) in tablets. A calibration set of 16 mixtures, each containing PAR and TRA in various amounts, was created using a 24-full fractional design. The absorbance data set for the calibration set were obtained between 215-280 nm ( increment & lambda; = 0.1 nm). Subsequently, the concentration and ab-sorbance sets were used to generate PCR and PLS calibrations. The ratio spectra-first derivative method was devised as a solution to the same problem to compare the outcomes of the chemometric methods used for the same experiment. After the proposed methods were shown to be accurate in the testing of validation samples, they were used in analysing commercial tablet samples. The analytical results showed that PCR and PLS methods can be used as alternative methods to high-performance liquid chromatography (HPLC). Correla-tion coefficients were determined for the working concentration range of 6-36 g.mL-1 for PAR and 4-22 g.mL-1 for TRA. The limits of detection and quantification were calculated as 0.9104 & mu;g.mL-1 and 3.0347 & mu;g.mL-1, respectively. The test results of the chemometric analyses and ratio spectra-first derivative method of the commercial tablet form are in agreement with the results of the one-way ANO-VA with a confidence interval of 95%. This study shows that the ratio spectra-first derivative method, PCR and PLS models based on spectrophotometric measurements are very useful and straightforward techniques for the quantitative resolution of a two-component pharmaceutical preparation, requiring little sample preparation and little time for analysis.en_US
dc.identifier.doi10.55730/1300-0527.3566
dc.identifier.endpage645en_US
dc.identifier.issn1300-0527
dc.identifier.issue3en_US
dc.identifier.pmid37529219en_US
dc.identifier.scopus2-s2.0-85164913340en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage633en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0527.3566
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16983
dc.identifier.volume47en_US
dc.identifier.wosWOS:001021568600010en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectParacetamolen_US
dc.subjectTramadol Hydrochlorideen_US
dc.subjectUv-Spectrophotometryen_US
dc.subjectChemometric Calibrationen_US
dc.titleSpectrophotometric quantification of paracetamol and tramadol hydrochloride by chemometric calibration methodsen_US
dc.typeArticleen_US

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