LOSS OF GIT1 GENE IN FISSION YEAST PROVIDES RESISTANCE TO ENDOPLASMIC RETICULUM STRESS

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dc.contributor.authorAkcaalan, Sedef
dc.contributor.authorPalabiyik, Bedia
dc.date.accessioned2024-02-23T14:49:15Z
dc.date.available2024-02-23T14:49:15Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractSchizosaccharomyces pombe detects glucose G-protein coupled receptor and transmits signal transduction via a cAMP-dependent protein kinase A (PICA) pathway. Also, Git1 C2 domain protein is required for maintaining basal cAMP levels and for producing glucose-triggered cAMP response. Actually, Git1 is a member of the UNC-13/Munc13 protein family that are conserved in eukaryotes and functionally included in synaptic vesicle exocytosis. We aimed to investigate the impact of Git1 on the vesicular trafficking dependent on endoplasmic reticulum and on the oxidative stress response. In this study, we cloned the full-length (git1T) of S. pombe girl gene into pSGP572 plasmid, as a S. pombe expression vector carrying thiamine repressed promoter, nmt1 and C-terminal GFP. Then, we compared the viability of S. pombe strains (wild type, git Delta, pSGP572/git Delta, pG1T/git Delta) against to H2O2-induced oxidative stress and tunicamycin-induced ER stress to that of their control groups. We found that deletion of git1 gene brought S. pombe resistance to ER stress, but not oxidative stress in S. pombe cells.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul University [23230]; Scientific and Technical Research Council of Turkey (TUBITAK) [116Z093]en_US
dc.description.sponsorshipWe thank to Umit Yasar KINA (Bezmialem University, TURKEY) for technical supports on confocal microscopy studies. This work was supported by Scientific Research Project Coordination Unit of Istanbul University, Project ID: 23230; by The Scientific and Technical Research Council of Turkey (TUBITAK), Project ID: 116Z093.en_US
dc.identifier.endpage9372en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue12en_US
dc.identifier.startpage9366en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/18067
dc.identifier.volume28en_US
dc.identifier.wosWOS:000503915900044en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSchizosaccharomyces Pombeen_US
dc.subjectGit1en_US
dc.subjectTunicamycinen_US
dc.subjectEr Stressen_US
dc.subjectOxidative Stressen_US
dc.titleLOSS OF GIT1 GENE IN FISSION YEAST PROVIDES RESISTANCE TO ENDOPLASMIC RETICULUM STRESSen_US
dc.typeArticleen_US

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