An electrochemical and computational study for discrimination of D- and L-cystine by reduced graphene oxide/?-cyclodextrin

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dc.contributor.authorZor, Erhan
dc.contributor.authorBingol, Haluk
dc.contributor.authorRamanaviciene, Almira
dc.contributor.authorRamanavicius, Arunas
dc.contributor.authorErsoz, Mustafa
dc.date.accessioned2024-02-23T14:16:45Z
dc.date.available2024-02-23T14:16:45Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractHere, we report a novel enantioselective electrochemical biosensor for the discrimination of cystine enantiomers (D- and L-cystine) using a chiral interface for the specific recognition of D- and L-cystine. The biosensor is based on reduced graphene oxide modified by beta-cyclodextrin (rGO/beta-CD) at the GCE surface. During the preparation of rGO/beta-CD/GCE, the modified electrode surfaces were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). The electrochemical behaviours of the D- and L-cystine were investigated using the rGO/beta-CD/GCE by CV and compared to bare GCE. A clear separation between the oxidation peak potentials of D- and L-cystine was observed at 1.32 and 1.42 V, respectively. The electrochemical discrimination performance of the fabricated chiral sensor was also examined by differential pulse voltammetry (DPV) in a mixed solution of D- and L-cystine. In addition, the DPV technique was used for the determination of D- and L-cystine at low concentration values in the range of 1.0-10.0 mM. To investigate the amperometric response of rGO/beta-CD/GCE towards D- and L-cystine, the chronoamperometry technique was used in the concentration range of 10.0-100.0 mu M. The interactions of the enantiomers with rGO/beta-CD were modelled by molecular docking using AutoDock Vina, and the interaction energies were predicted to be -4.8 and -5.3 kcal mol(-1) for D- and L-cystine, respectively. The corresponding values of binding constants were calculated to be 3.32 x 10(3) and 7.71 x 10(3) M-1, respectively. The experimental and molecular docking results indicate that the rGO/beta-CD/GCE has a different affinity for each enantiomer.en_US
dc.description.sponsorshipTUBITAK [113Z664]en_US
dc.description.sponsorshipThis work was supported by TUBITAK (113Z664) and was produced from a part of E. Zor's PhD thesis. We also express our deep thanks to the Turkish Academy of Sciences (TUBA).en_US
dc.identifier.doi10.1039/c4an01751j
dc.identifier.endpage321en_US
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue1en_US
dc.identifier.pmid25382195en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage313en_US
dc.identifier.urihttps://doi.org/10.1039/c4an01751j
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12776
dc.identifier.volume140en_US
dc.identifier.wosWOS:000345824700037en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalysten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleAn electrochemical and computational study for discrimination of D- and L-cystine by reduced graphene oxide/?-cyclodextrinen_US
dc.typeArticleen_US

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