Determination of the target proteins in chemotherapy resistant breast cancer stem cell-like cells by protein array

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dc.contributor.authorKars, Meltem Demirel
dc.contributor.authorYildirim, Gamze
dc.date.accessioned2024-02-23T14:02:45Z
dc.date.available2024-02-23T14:02:45Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractBreast cancer comes second among the causes of cancer deaths of women. Although new generation hormone therapy is a promising strategy, re-occurrence or emergence of drug resistance limits the success. According to the theory of cancer stem cells (CSCs); CSCs are immortal, tumor inducing and self renewing pluripotent cells and multiply as chemotherapy proceeds, making the chemotherapy inefficient. Emerging scientific reports indicate that the mechanisms of drug resistance are the main features that CSCs gain actually. Due to this fact, cancer stem cell markers should be clarified to target CSCs and this will play important role to reverse drug resistance. In this study, MCF-7 /Pac, a cell line resistant to microtubule inhibitor paclitaxel and multiple drugs permanently, was used as a reference cell line for drug resistant mammary cancer. It has some properties that breast cancer stem cells possess so it is considerable to isolate breast cancer stem cell-like cells from MCF-7 /Pac population. The chemotherapy resistant breast cancer stem-like (BCSC-like) cells were sorted from MCF-7 /Pac population by using markers CD44, CD24 and ALDH. At the next step the proteins that are up-regulated in BCSC-like cells were determined by protein array analysis. Additionally the effect of paclitaxel on BCSC-like cell proliferation was determined. The MCF-7 /Pac population contains 12.4% BCSC-like cells. The cells bearing BCSC-like cell phenotype exhibited resistance to paclitaxel. The over-expressed growth factors, MMP proteins, Frizzled proteins and IL-23 were found to be related to the BCSC-like cell proliferation. These results will guide both basic science and medical science.en_US
dc.description.sponsorshipSelcuk University, BAP project [14401034]en_US
dc.description.sponsorshipThis study was supported by Selcuk University, BAP project (project no: 14401034), Turkey.en_US
dc.identifier.doi10.1016/j.ejphar.2019.01.052
dc.identifier.endpage29en_US
dc.identifier.issn0014-2999
dc.identifier.issn1879-0712
dc.identifier.pmid30707960en_US
dc.identifier.scopus2-s2.0-85060875515en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage23en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejphar.2019.01.052
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11836
dc.identifier.volume848en_US
dc.identifier.wosWOS:000459483200003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal Of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast Cancer Stem Cellen_US
dc.subjectProtein Arrayen_US
dc.subjectDrug Resistanceen_US
dc.subjectFrizzleden_US
dc.subjectAldhen_US
dc.subjectCd44en_US
dc.titleDetermination of the target proteins in chemotherapy resistant breast cancer stem cell-like cells by protein arrayen_US
dc.typeArticleen_US

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