Electrochemical Glucose Biosensors: Whole Cell Microbial and Enzymatic Determination Based on 10-(4H-Dithieno[3,2-b:2?,3?-d]Pyrrol-4-yl)Decan-1-Amine Interfaced Glassy Carbon Electrodes

Basit Öğe Kaydı
dc.contributor.authorCevik, Emre
dc.contributor.authorCerit, Alaaddin
dc.contributor.authorTombuloglu, Huseyin
dc.contributor.authorSabit, Hussein
dc.contributor.authorYildiz, Huseyin Bekir
dc.date.accessioned2024-02-23T14:17:10Z
dc.date.available2024-02-23T14:17:10Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractThe fabrication of amperometric biosensors based on whole cell Gluconobacter oxydans DSMZ 2343 (G. oxydans) and glucose oxidase (GOx) was performed for the detection of glucose. Glassy carbon electrodes (GCE) were coated with a 10-(4H-dithiyeno [3,2-b:2',3'-d]pyroll-4-il)decan-1-amine (DTP-alkyl-NH2) polymer using an electropolymerization method and the formed interface was used to connect the bacteria and the enzyme to the electrode. The transfer of electrons from enzyme to electrode was successfully demonstrated by the biocatalytic activity and unique morphology of the conducting polymer. Characterization of the biosensors was assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM) analyses. The detection limits of the enzyme and microbial based biosensors for glucose were 0.022 and 0.081 mM, respectively. The broad linear dynamic ranges of the GOx and G. oxydans biosensors were observed to be 0.045-50.0 and 0.19-50.0 mM, respectively. The analytical performances of biosensors were compared according to the following figures of merit: detection limits, limits of quantification, pH and current response time. In addition, to demonstrate the applicability of the biosensors, real-time measurements and recovery studies were evaluated.en_US
dc.description.sponsorshipEuropean Union through the COST Action [CM1104]; Scientific and Technological Research Council of Turkey (TUBITAK) [112T622]en_US
dc.description.sponsorshipThe authors would like to thank the European Union through the COST Action CM1104 Reducible Oxide Chemistry, Structure and Functions and the Scientific and Technological Research Council of Turkey (TUBITAK Grant Number 112T622) for the financial support of this work.en_US
dc.identifier.doi10.1080/00032719.2018.1521828
dc.identifier.endpage1152en_US
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85055580370en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1138en_US
dc.identifier.urihttps://doi.org/10.1080/00032719.2018.1521828
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12953
dc.identifier.volume52en_US
dc.identifier.wosWOS:000461872800008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucose Biosensoren_US
dc.subjectGlucose Oxidaseen_US
dc.subjectG. Oxydansen_US
dc.subject10-(4h-Dithiyeno[3,2-B,2 ',3 '-D]Pyroll-4-Il)Decan-1-Amine (Dtp) Conducting Polymeren_US
dc.subjectWhole Cell Microbial Biosensoren_US
dc.titleElectrochemical Glucose Biosensors: Whole Cell Microbial and Enzymatic Determination Based on 10-(4H-Dithieno[3,2-b:2?,3?-d]Pyrrol-4-yl)Decan-1-Amine Interfaced Glassy Carbon Electrodesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu