Assessment of genetic diversity and population structure in local alfalfa genotypes using iPBS molecular markers

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dc.contributor.authorEren, Baris
dc.contributor.authorKeskin, Bilal
dc.contributor.authorDemirel, Fatih
dc.contributor.authorDemirel, Serap
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorYilmaz, Abdurrahim
dc.contributor.authorHaliloglu, Kamil
dc.date.accessioned2024-02-23T13:56:09Z
dc.date.available2024-02-23T13:56:09Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractiPBS retrotransposon markers, in other words, inter-primer binding site markers based on retrotransposon, have been helpful for the determination of genetic diversity in several plants. The study was evaluated using 10 iPBS molecular markers on the level of genetic diversity and genetic structure of alfalfa genotypes. A total of 280 alleles in 50 alfalfa genotypes (48 local genotypes and 2 commercial varieties) were obtained by 267 polymorphic markers with an average of 28 per locus, ranging from 9 to 41 alleles. The rate of polymorphism of the markers ranged from 86.36 to 100% with an average of 93.71%. The average number of polymorphic bands per marker was detected as 26.7. The mean PIC value and Dice's similarity index were calculated as 0.14 and 0.50 respectively. The results of UPGMA analysis, principal coordinate analysis (PCoA), and STRUCTURE demonstrated that the 50 alfalfa genotypes could be classified into 4 subpopulations, namely the Q1, Q2, Q3, and Q4. The Nei's genetic distances ranged from 0.0121 to 0.0359. iPBS markers and alfalfa genotypes used in this study may be used in studies of alfalfa breeding and germplasm conservation.en_US
dc.description.sponsorshipIgdir University [2017-FBE-A02]en_US
dc.description.sponsorshipThis study is a part of PhD research and was financially supported by Igdir University with the project number 2017-FBE-A02.en_US
dc.identifier.doi10.1007/s10722-022-01450-2
dc.identifier.endpage628en_US
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85136119954en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage617en_US
dc.identifier.urihttps://doi.org/10.1007/s10722-022-01450-2
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11096
dc.identifier.volume70en_US
dc.identifier.wosWOS:000841142400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofGenetic Resources And Crop Evolutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMedicago Sativaen_US
dc.subjectMolecular Characterizationen_US
dc.subjectPre-Breedingen_US
dc.subjectPopulation Structureen_US
dc.subjectUpgmaen_US
dc.titleAssessment of genetic diversity and population structure in local alfalfa genotypes using iPBS molecular markersen_US
dc.typeArticleen_US

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