Biomarkers to target and silence stemness of breast cancer stem cell model: silencing MDR1 by siRNA

Basit Öğe Kaydı
dc.contributor.authorYildirim, Gamze
dc.contributor.authorKars, Meltem D.
dc.contributor.authorKars, Gokhan
dc.contributor.authorKilic, Hamdi S.
dc.date.accessioned2024-02-23T14:31:54Z
dc.date.available2024-02-23T14:31:54Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractObjectives Aim of the study was to reveal new biomarker genes to target breast cancer stem-like cells (BCSC-like) and then sensitize BCSC-like cells to chemotherapy by silencing MDR1 gene found to be the most suitable target. Methods Drug resistance associated genes were screened by cDNA microarray to unveil biomarker genes in drug resistance breast cancer model cells. Drug resistance was then reversed by silencing MDR1 gene in BCSC-like cells. The effect of silencing was monitored by real-time cell proliferation analysis. Differential expressions of MDR1, ALDH1A3, EGFR and BAG4 genes were identified by real-time PCR. P-glycoprotein (P-gp) expression level and its activity were investigated by Western blot and flow cytometry measurements, respectively. Results 16 new biomarker genes were identified upon gene expression analysis by cDNA microarray. MDR1 gene was selected as the most potent target gene and silencing of it caused down-regulation of MDR1, ALDH1A3, EGFR, BAG4 expression and P-glycoprotein activity and expression in BCSC-like cells. At the end, silenced BCSC-like cells were found to be more responsive to paclitaxel therapy. Conclusions In conclusion, siMDR1 silencing is an effective way to reverse multidrug resistance and malignancy. New biomarker genes revealed in this study require to be investigated to target stemness of BC.en_US
dc.description.sponsorshipSelcuk University [15201105]en_US
dc.description.sponsorshipThe project was funded by Selcuk University (project number: 15201105).en_US
dc.identifier.doi10.1515/tjb-2021-0275
dc.identifier.endpage455en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85146395314en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage445en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2021-0275
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15406
dc.identifier.volume47en_US
dc.identifier.wosWOS:000773666400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast Cancer Stem Cellsen_US
dc.subjectEgfren_US
dc.subjectMicroarrayen_US
dc.subjectPaclitaxelen_US
dc.subjectSimdr1en_US
dc.titleBiomarkers to target and silence stemness of breast cancer stem cell model: silencing MDR1 by siRNAen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu