The trnL (UAA)-trnF (GAA) intergenic spacer is a robust marker of green pea (Pisum sativum L.) adulteration in economically valuable pistachio nuts (Pistacia vera L.)

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dc.contributor.authorSen, Fatima
dc.contributor.authorUncu, Ayse Ozgur
dc.contributor.authorUncu, Ali Tevfik
dc.contributor.authorErdeger, Seyma Nur
dc.date.accessioned2024-02-23T12:17:18Z
dc.date.available2024-02-23T12:17:18Z
dc.date.issued2020
dc.departmentNEÜen_US
dc.description.abstractBACKGROUND Pistachio (Pistacia vera L.) is an expensive culinary nut species; it is therefore susceptible to adulteration for economic profit. Green pea (Pisum sativum L.) kernels constitute the most common material used for adulterating chopped / ground pistachio nuts and pistachio paste. Food genomics enables the species composition of a food sample to be ascertained through DNA analysis. Accordingly, a barcode DNA genotyping approach was used to standardize a test method to identify green pea adulteration in pistachio nuts. RESULTS The trnL (UAA)-trnF (GAA) intergenic spacer in the plastid genome was the target analyte in the present study. The barcode locus displayed a significant, discriminatory size difference between pistachio and pea, with amplicon sizes of 449 and 179 bp, respectively. Polymerase chain reaction-capillary electrophoresis (PCR-CE) analysis of the intergenic spacer resulted in the successful identification of species composition in the in-house admixtures, which contained 5% to 30% of green pea. CONCLUSION The present work describes a fast and straightforward DNA test that identifies green pea adulteration in pistachio nuts without requiring a statistical data interpretation process. The plastid trnL (UAA)-trnF (GAA) intergenic spacer length widely varies among plant taxa, so the PCR-CE protocol that operates on the intergenic spacer holds the potential to reveal adulteration with a plethora of adulterants. The PCR-CE assay described in the present work can be adopted readily by food-quality laboratories in the public sector or the food industry as an easy and reliable method to analyze pistachio authenticity. (c) 2020 Society of Chemical Industryen_US
dc.identifier.doi10.1002/jsfa.10336
dc.identifier.endpage3061en_US
dc.identifier.issn0022-5142
dc.identifier.issn1097-0010
dc.identifier.issue7en_US
dc.identifier.pmid32077485en_US
dc.identifier.scopus2-s2.0-85080128165en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage3056en_US
dc.identifier.urihttps://doi.org/10.1002/jsfa.10336
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10590
dc.identifier.volume100en_US
dc.identifier.wosWOS:000516635200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of The Science Of Food And Agricultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCapillary Electrophoresisen_US
dc.subjectChloroplast Genomeen_US
dc.subjectDna Barcodeen_US
dc.subjectFood Genomicsen_US
dc.subjectPistachioen_US
dc.titleThe trnL (UAA)-trnF (GAA) intergenic spacer is a robust marker of green pea (Pisum sativum L.) adulteration in economically valuable pistachio nuts (Pistacia vera L.)en_US
dc.typeArticleen_US

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