IN VITRO SCREENING OF PRECONDITIONED PLUMULAR APICES EXPLANTS OF PEANUT (ARACHIS HYPOGEAE) TO DIFFERENT SALTS CONCENTRATION

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dc.contributor.authorDay, Sibel
dc.contributor.authorAasim, Muhammad
dc.date.accessioned2024-02-23T14:49:04Z
dc.date.available2024-02-23T14:49:04Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractHigh salinity level in soil or irrigation water limits the plant growth and yield due to altering the metabolic processes, enzymes contents and activities. Peanut is sensitive plant to salinity and salinity level above EC of 8 dS m(-1) which hindered the germination rate and plant growth. In vitro screening method using plant tissue culture techniques could be employed to identify salt tolerance of explant, cultivar or plant without exposing to soil or climate. In this study, preconditioned plumular apices explants were cultured on agar solidified MS medium suplemented with 1.0 mg l(-1) BAP and 5-20 pS cm(-1) salinity level of NaCl and KCl for two months. 100% callus induction and shoot regeneration frequency was recorded along with browning or necrosis on callus or regnerated shoots on all mediums irrespective of salty type or concentration. Increased salt concentrations significally inhibited the number of shoots and shoot length compared to control. Regenerated shoots (<= 0.5 cm) were rooted on rooting medium enriched with 0.25-2.0 mg l(-1) IBA or 1.0 mg l(-1) IBA with 0.15-0.6% sucrose concentrations. Rooted plantlets were successfully acclimatized in the pots containing peat moss or coarse sand: peat moss soil mix (1:1) in the growth room. Results revealed that preconditioned plumular explants can be used for modern biotechnological techniques like in vitro selection or genetic transformation studies.en_US
dc.identifier.endpage4676en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue7en_US
dc.identifier.startpage4671en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/17953
dc.identifier.volume26en_US
dc.identifier.wosWOS:000406355800046en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn Vitroen_US
dc.subjectKclen_US
dc.subjectNaclen_US
dc.subjectSalinityen_US
dc.subjectEcen_US
dc.subjectBapen_US
dc.subjectPreconditioningen_US
dc.titleIN VITRO SCREENING OF PRECONDITIONED PLUMULAR APICES EXPLANTS OF PEANUT (ARACHIS HYPOGEAE) TO DIFFERENT SALTS CONCENTRATIONen_US
dc.typeArticleen_US

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