Synthesis and Characterization of Chitosan/TiO2 Composite Beads for Improving Stability of Porcine Pancreatic Lipase

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dc.contributor.authorDeveci, Ilyas
dc.contributor.authorDogac, Yasemin Ispirli
dc.contributor.authorTeke, Mustafa
dc.contributor.authorMercimek, Bedrettin
dc.date.accessioned2024-02-23T13:59:42Z
dc.date.available2024-02-23T13:59:42Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractThe purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads. The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v). The optimum temperature (20-60 A degrees C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 A degrees C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system. The optimum temperatures of free and immobilized lipase were 30 A degrees C. The temperature profile of the immobilized lipase was spread over a large area. The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively. The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 A degrees C. But, at this temperature, soluble lipase protected only 15 % activity. Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR). The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.en_US
dc.identifier.doi10.1007/s12010-014-1321-4
dc.identifier.endpage1068en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue2en_US
dc.identifier.pmid25359676en_US
dc.identifier.scopus2-s2.0-84921061964en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1052en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-014-1321-4
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11286
dc.identifier.volume175en_US
dc.identifier.wosWOS:000348103100036en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofApplied Biochemistry And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLipaseen_US
dc.subjectImmobilizationen_US
dc.subjectChitosanen_US
dc.subjectTio2en_US
dc.subjectComposite Beaden_US
dc.titleSynthesis and Characterization of Chitosan/TiO2 Composite Beads for Improving Stability of Porcine Pancreatic Lipaseen_US
dc.typeArticleen_US

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