The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

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dc.contributor.authorTurkoglu, Aras
dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorBalpinar, Ozge
dc.contributor.authorOzturk, Halil Ibrahim
dc.contributor.authorOzkan, Guller
dc.contributor.authorPoczai, Peter
dc.date.accessioned2024-02-23T14:35:19Z
dc.date.available2024-02-23T14:35:19Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractMammalian sex hormones are steroid-structured compounds that support the growth and development of plants at low concentrations. Since they affect the physiological processes in plants, it has been thought that mammalian sex hormones may cause modifications to plant genomes and epigenetics. This study aims to determine whether different mammalian sex hormones (17 beta-estradiol, estrogen, progesterone, and testosterone) in several concentrations (0, 10(-4), 10(-6), and 10(-8) mM) affect genetic or epigenetic levels in bean plants, using in vitro tissue cultures from plumule explants. We investigated levels of DNA damage, changes in DNA methylation and DNA stability in common bean exposed to mammalian sex hormones (MSH) using inter-primer binding site (iPBS) and Coupled Restriction Enzyme Digestion-iPBS (CRED-iPBS) assays, respectively. The highest rate of polymorphism in iPBS profiles was observed when 10(-4) mM of estrogen (52.2%) hormone was administered. This finding indicates that genetic stability is reduced. In the CRED-iPBS profile, which reveals the methylation level associated with the DNA cytosine nucleotide, 10(-4) mM of estrogen hormone exhibited the highest hypermethylation value. Polymorphism was observed in all hormone administrations compared to the control (without hormone), and it was determined that genomic stability was decreased at high concentrations. Taken together, the results indicate that 17 beta-estradiol, estrogen, progesterone, and testosterone in bean plants affect genomic instability and cause epigenetic modifications, which is an important control mechanism in gene expression.en_US
dc.description.sponsorshipEotvos Research Fund; Helsinki University Libraryen_US
dc.description.sponsorshipP.P. expresses his gratitude for the support of the Eotvos Research Fund. The authors thank the Helsinki University Library for supporting open-access publication.en_US
dc.identifier.doi10.3390/plants11152071
dc.identifier.issn2223-7747
dc.identifier.issue15en_US
dc.identifier.pmid35956548en_US
dc.identifier.scopus2-s2.0-85136987563en_US
dc.identifier.urihttps://doi.org/10.3390/plants11152071
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15950
dc.identifier.volume11en_US
dc.identifier.wosWOS:000839001800001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPlants-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCred-Ipbsen_US
dc.subjectEpigeneticsen_US
dc.subjectGenotoxicen_US
dc.subjectIn Vitroen_US
dc.titleThe Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)en_US
dc.typeArticleen_US

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