3?,4?-Dihydroxyflavonol (DiOHF) prevents DNA damage, lipid peroxidation and inflammation in ovarian ischaemia-reperfusion injury of rats

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dc.contributor.authorAgartan, Elif Sena
dc.contributor.authorMogulkoc, Rasim
dc.contributor.authorBaltaci, Abdulkerim Kasim
dc.contributor.authorMenevse, Esma
dc.contributor.authorDasdelen, Dervis
dc.contributor.authorAvunduk, Mustafa Cihat
dc.date.accessioned2024-02-23T14:17:13Z
dc.date.available2024-02-23T14:17:13Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractThis study aimed to determine the effect of 3',4'-Dihydroxyflavonol (DiOHF) on lipid peroxidation, DNA damage and inflammation in ovarian ischaemia (I)-reperfusion (R) injury. This study was performed on 44 Wistar-albino female rats. Groups were designed as Control; Sham; I/R (the left ovary was ligated for 2 h and then reperfused for 2 h); I/R + DiOHF (after 2 h ischaemia and 2 h reperfusion, 30 mg/kg of DiOHF was given intraperitoneally and reperfusion was allowed for 2 h more); I + DiOHF + R (after 2 h I, 30 mg/kg of DiOHF was given at the beginning of 2 h reperfusion); DiOHF + I/R (2 h after DiOHF administration, the left ovary was ligated for 2 h and then reperfused for 2 h). Blood and ovarian tissue samples were analysed for GSH, MDA, 8-OHdG, SOD, and IL-6. Ovarian tissue was examined histopathologically. Ovarian I/R has led to inflammation and oxidative damage. However, DiOHF activated the antioxidant system and prevented DNA damage induced by I/R in ovarian tissue. Vascularisation, oedema, and inflammation also occurred in ovarian tissue in I/R group. The results of this study indicated that I/R led to disturbance of the oxidant/antioxidant system balance and increased DNA damage; however, DiOHF supplementation prevented DNA damage, lipid peroxidation and inflammation by increasing the antioxidant system in ovarian I/R injury in rats. However, in potential I/R situations, DiOHF application appears to be beneficial in reducing inflammation, oxidant injury, and DNA damage, and in activating the antioxidant system. IMPACT STATEMENT What is already known on this subject? Ischaemia/reperfusion (I/R) injuries lead to damage in cells or tissues due to insufficient blood flow. What do the results of this study add? Increased DNA injury and inflammatory response (IL-6) and structural impairment were treated by administration of intraperitoneal (DiOHF) which strongly stimulated the antioxidant system, inhibited antioxidant activities, prevented DNA damage and inflammation process. What are the implications of these findings for clinical practice and/or further research? This study's strength is that it is the first research demonstrates the prevention of DNA damage in ovarian I/R by DiOHF supplementation. This flavonoid (DiOHF) may be used for treatment in different ovarian ischaemia/reperfusion.en_US
dc.description.sponsorshipSelcuk University, Scientific Research Council [16202011]en_US
dc.description.sponsorshipThis study was supported by a grant from Selcuk University, Scientific Research Council [Grant number is 16202011].en_US
dc.identifier.doi10.1080/01443615.2021.1916813
dc.identifier.endpage345en_US
dc.identifier.issn0144-3615
dc.identifier.issn1364-6893
dc.identifier.issue2en_US
dc.identifier.pmid34159896en_US
dc.identifier.scopus2-s2.0-85108368891en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage338en_US
dc.identifier.urihttps://doi.org/10.1080/01443615.2021.1916813
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12997
dc.identifier.volume42en_US
dc.identifier.wosWOS:000664892400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal Of Obstetrics And Gynaecologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOvarian Ischaemiaen_US
dc.subjectInterleukin-6 (Il-6)en_US
dc.subjectGlutathione (Gsh)en_US
dc.subjectMalondialdehyde (Mda)en_US
dc.subject8-Hydroxydeoxyguanosine (8-Ohdg)en_US
dc.subjectSuperoxide Dismutase (Sod)en_US
dc.subjectDna Damageen_US
dc.title3?,4?-Dihydroxyflavonol (DiOHF) prevents DNA damage, lipid peroxidation and inflammation in ovarian ischaemia-reperfusion injury of ratsen_US
dc.typeArticleen_US

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