Evaluation of Semen Samples Before and After 'Swim Up' Technique with Mitotracker

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dc.contributor.authorSeda, Cetinkaya Karabekir
dc.contributor.authorOzgorgulu, Aydan
dc.date.accessioned2024-02-23T14:34:51Z
dc.date.available2024-02-23T14:34:51Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractObjectives: Infertility problems are seen in nearly 15% of married couples, and almost 50% of these involve male infertility. The sperm mitochondrion plays an important role in sperm motility. The morphology of sperm, sperm preparation techniques, and the number and motility of sperm during insemination are important parameters that affect the rate of pregnancy with intrauterine insemination. The aim of the study was to observe the changes in sperm mitochondrial activities and numbers with the Mito Tracker Red 580 kit at 30th, 45th and 60th minutes after swim up. Materials and Methods: In this study, we evaluated 20 oligospermic and 20 normospermic samples. The swim-up technique was applied to these oligospermic and normospermic samples, and changes in sperm mitochondria and number at 30, 45, and 60 min were observed using MitoTracker Red 580 staining. Results: The numbers of swimming sperm in oligospermic and normospermic samples were higher at 30 min than at 45 and 60 min. No marked change was detected in the mitochondrial activities of swimming sperm in oligospermic and normospermic samples at 30, 45, and 60 min after the swim-up. Our findings demonstrate that the number of swimming sperm decreased with time, but no change was seen in the mitochondrial activity of the sperm by the swim-up technique. Conclusion: According to findings, mitochondrial movement is not exactly related to the 30th, 45th and 60th minutes. Therefore, it is thought that it needs to be investigated in mechanisms other than mitochondrial activity.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit Supporting Protocol of University of Necmettin Erbakanen_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Coordination Unit Supporting Protocol of University of Necmettin Erbakan. We appreciate this support.en_US
dc.identifier.doi10.3329/bjms.v18i3.41614
dc.identifier.endpage483en_US
dc.identifier.issn2223-4721
dc.identifier.issn2076-0299
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85068006736en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage479en_US
dc.identifier.urihttps://doi.org/10.3329/bjms.v18i3.41614
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15766
dc.identifier.volume18en_US
dc.identifier.wosWOS:000469991300005en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIbn Sina Trusten_US
dc.relation.ispartofBangladesh Journal Of Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSpermen_US
dc.subjectSwim-Up Methoden_US
dc.subjectMitotracker 580en_US
dc.subjectOligospermiaen_US
dc.titleEvaluation of Semen Samples Before and After 'Swim Up' Technique with Mitotrackeren_US
dc.typeArticleen_US

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