Biofilm formation by Staphylococcus aureus isolates from a dental clinic in Konya, Turkey

Basit Öğe Kaydı
dc.contributor.authorTorlak, Emrah
dc.contributor.authorKorkut, Emre
dc.contributor.authorUncu, Ali T.
dc.contributor.authorSener, Yagmur
dc.date.accessioned2024-02-23T14:12:50Z
dc.date.available2024-02-23T14:12:50Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractThe ability of Staphylococcus aureus to form biofilm is considered to be a major virulence factor influencing its survival and persistence in both the environment and the host. Biofilm formation in S. aureus is most frequently associated with production of polysaccharide intercellular adhesion by ica operon-encoded enzymes. The present work aimed at evaluating the in vitro biofilm production and presence of the icaA and icaD genes in S. aureus isolates from a dental clinic in Konya, Turkey. The surfaces of inanimate objects were sampled over a period of six months. S. aureus isolates were subjected to Congo Red Agar (CRA) and crystal violet (CV) staining assays to evaluate their ability of biofilm production, while the presence of the icaA and icaD genes was determined by polymerase chain reaction. S. aureus contamination was detected in 13.2% of the environmental samples. All the 32 isolates were observed to be positive for both the icaA and icaD genes. Phenotypic evaluations revealed that CV staining assay is a more reliable alternative to CRA assay to determine biofilm formation ability. A high percentage of agreement (91%) was observed between the results from CV staining and ica genes' detection assays. Phenotypic and genotypic evaluations should be combined to detect biofilm formation in S. aureus. Our findings indicate that dental clinic environments should be considered as potential reservoir for biofilm-producing S. aureus and thus cross contamination. (C) 2017 The Authors. Published by Elsevier Limited.en_US
dc.identifier.doi10.1016/j.jiph.2017.01.004
dc.identifier.endpage813en_US
dc.identifier.issn1876-0341
dc.identifier.issn1876-035X
dc.identifier.issue6en_US
dc.identifier.pmid28214190en_US
dc.identifier.scopus2-s2.0-85012940522en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage809en_US
dc.identifier.urihttps://doi.org/10.1016/j.jiph.2017.01.004
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12211
dc.identifier.volume10en_US
dc.identifier.wosWOS:000414231900023en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Londonen_US
dc.relation.ispartofJournal Of Infection And Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStaphyloccocus Aureusen_US
dc.subjectDental Clinic Surfacesen_US
dc.subjectBiofilmen_US
dc.subjectIca Genesen_US
dc.subjectKonyaen_US
dc.titleBiofilm formation by Staphylococcus aureus isolates from a dental clinic in Konya, Turkeyen_US
dc.typeArticleen_US

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