Antioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damage

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dc.contributor.authorAyla, Sule
dc.contributor.authorTunali, Gulden
dc.contributor.authorBilgic, Bulent E.
dc.contributor.authorSofuoglu, Kenan
dc.contributor.authorOzdemir, A. Arman
dc.contributor.authorTanriverdi, Gamze
dc.contributor.authorOzdemir, Semra
dc.date.accessioned2024-02-23T14:00:16Z
dc.date.available2024-02-23T14:00:16Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractPurpose: Sperm processing (e.g., centrifugation) used in preparation for assisted reproduction can result in excessive generation of reactive oxygen species (ROS) and potential sperm damage. The use of antioxidants during sperm processing has been shown to prevent iatrogenic sperm damage, including DNA damage. In this study, we evaluated the effect of caffeic acid phenethyl ester (CAPE) on oxidative stress mediated sperm dysfunction and DNA damage. Methods: Semen samples were obtained to liquefy at room temperature. After centrifugation and washing protocols, spermatozoa were incubated in a single step supplemented medium with either of 10, 50 or 100 mu mol/L CAPE for 2 hours at 36 degrees C. After incubation period, MDA levels of seminal plasma were measured. The fragmentation in sperm DNA was detected by light microscopy via use of an aniline blue assay, while ultrastructural morphology was analyzed by transmission electron microscopy. Results: Significant increase has been observed in percent chromatin condensation (assessed by aniline blue staining) and Malondialdehyde (Mmol/L) in oligoasthenoteratozoospermia group before the centrifugation (0.57 +/- 0.15). Incubation of samples with 100 mu mol/L CAPE after centrifugation resulted in a significantly lower percent chromatin condensation compared to samples incubated without CAPE (0.42 +/- 0.12) (P < 0.0033). Incubation of all samples with CAPE (10 mu mol/L, 50 mu mol/L, 100 mu mol/L.) after centrifugation resulted in a significantly lower percentage of Malondialdehyde levels. Conclusions: The data suggests that preincubation of spermatozoa with the antioxidant CAPE offers protection against oxidative DNA damage in vitro.en_US
dc.description.sponsorshipIstanbul University, Cerrahpasa Medical Faculty, Department of Histology and Embryology, Electron Microscopy Laboratoryen_US
dc.description.sponsorshipThe authors would like to thank Istanbul University, Cerrahpasa Medical Faculty, Department of Histology and Embryology, Electron Microscopy Laboratory for the support of TEM analysis of this study.en_US
dc.identifier.doi10.1016/j.acthis.2018.01.001
dc.identifier.endpage121en_US
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.issue2en_US
dc.identifier.pmid29325972en_US
dc.identifier.scopus2-s2.0-85044262029en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage117en_US
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2018.01.001
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11534
dc.identifier.volume120en_US
dc.identifier.wosWOS:000429186500007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbh, Urban & Fischer Verlagen_US
dc.relation.ispartofActa Histochemicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSperm Dna Damageen_US
dc.subjectAntioxidanten_US
dc.subjectCapeen_US
dc.subjectSperm Motilityen_US
dc.titleAntioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damageen_US
dc.typeArticleen_US

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