The Effects of Adipose-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cell-Originating Exosomes on Nerve Allograft Regeneration An Experimental Study in Rats

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dc.contributor.authorKoplay, Tugba Gun
dc.contributor.authorYildiran, Gokce
dc.contributor.authorDursunoglu, Duygu
dc.contributor.authorAktan, Murad
dc.contributor.authorDuman, Selcuk
dc.contributor.authorAkdag, Osman
dc.contributor.authorKaramese, Mehtap
dc.date.accessioned2024-02-23T14:23:27Z
dc.date.available2024-02-23T14:23:27Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractIntroductionNerve regeneration has been the subject of many studies because of its complex mechanism and functional outcome. Mesenchymal stem cells and exosomes are promising factors in regeneration in many areas. Reconstruction of nerve defects is a controversial issue, and nerve allografts are promising alternatives with many advantages. In this study, it is aimed to evaluate the nerve regeneration in cellularized and decellularized nerve allografts and whether it is possible to accelerate this process with adipose-derived mesenchymal stem cells (ad MSC) or ad MSC-originating exosomes.MethodThis study was performed with 36 Lewis and 18 Brown Norway isogenic male rats aged 10 to 12 weeks and weighing 300 to 350 g. The Lewis rats were divided into 6 groups. Nerve allografts at a length of 12 mm that were obtained from the Brown Norway rats' proximal portion of both sciatic nerve branching points were coapted as cellularized in group A and decellularized in group B to the sciatic nerve defects of the Lewis rats. Group A received oral tacrolimus (0.2 mg/kg) for 30 days. Perineural saline (A1-B1), ad MSC (A2-B2), or ad MSC-originating exosomes (A3-B3) were applied to these groups. Walking track analysis, pinch-prick test and electromyelography were applied at the 8th and 16th weeks following surgery. Nerves were examined histopathologically at the 16th week.ResultsBetween cellularized groups, better results were shown in A3 about axon-myelin regeneration/organization (P = 0.001), endoneural connective tissue (P = 0.005), and inflammation (P = 0.004). Better results were shown in the B2 and B3 groups electromyelographicaly about latency period (P = 0.033) and action potential (P = 0.008) at late period, and histomorphologicaly at vascularization (P = 0.012).DiscussionIt is argued that regeneration is accelerated with decellularization of nerve allografts by removing the chondroidin sulfate proteoglycans. The positive effects of stem cells are derived by exosomes without the cell-related disadvantages. In this study, better results were obtained by decellularization and perineural application of ad MSC and/or ad MSC exosome.en_US
dc.identifier.doi10.1097/SAP.0000000000003414
dc.identifier.endpage266en_US
dc.identifier.issn0148-7043
dc.identifier.issn1536-3708
dc.identifier.issue3en_US
dc.identifier.pmid36796049en_US
dc.identifier.scopus2-s2.0-85148252988en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage261en_US
dc.identifier.urihttps://doi.org/10.1097/SAP.0000000000003414
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13551
dc.identifier.volume90en_US
dc.identifier.wosWOS:000935975600013en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofAnnals Of Plastic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectExosomeen_US
dc.subjectNerve Allograften_US
dc.subjectNerve Regenerationen_US
dc.subjectStem Cellen_US
dc.titleThe Effects of Adipose-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cell-Originating Exosomes on Nerve Allograft Regeneration An Experimental Study in Ratsen_US
dc.typeArticleen_US

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