Determination of antibiotic resistance and high-performance liquid chromatography profiles for Mycobacterium species

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dc.contributor.authorOzer, Turkan Toka
dc.contributor.authorYula, Erkan
dc.contributor.authorDogan, Metin
dc.contributor.authorBaskin, Huseyin
dc.date.accessioned2024-02-23T12:11:10Z
dc.date.available2024-02-23T12:11:10Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractBackgroundIncidence of mycobacterial infections has been increasing. However, diagnosis and treatment of mycobacterial infections can be difficult. The aim of this study was to investigate high-performance liquid chromatography (HPLC) analysis of the mycolic acids for rapid identification and dendrogram cluster analysis of mycobacterium species. MethodsClinical specimens received for mycobacterial culture and antimicrobial susceptibility test were processed by standard laboratory protocols. Positive cultures were analyzed with HPLC method. Mycolic acid analysis with HPLC was used for diagnosis of tuberculosis and other mycobacterial infections. These reports were compared with Sherlock Library mycobacterial species, and the similarity index was analyzed. This value was formed by a software in multidimensional space that was the calculation of the average distance between the nearest library profile and unknown profile. ResultsThe ninety-two samples were identified as M.tuberculosis. (similarity index between 0.593 and 0.994). One of the other strains was identified as M.avium intracellulare (strain No. 82) (SI=0.906); one of them was identified as M.interjectum (strain no. 89) (SI=0.644). Total 94 samples were identified, and dendrogram was applied to these samples. Profile A (10.6%), profile B (59.6%), profile C (11.7%), profile D (3.2%), and other profiles as single different profiles were identified. Rates for each as 1% (89, 94, 1, 82, 26, 42, 32, 41, 100, 43, 47, 44, 40, 35). ConclusionHigh-performance liquid chromatography is a useful, rapid, reliable, and practical method for diagnosis of mycobacterium species.en_US
dc.description.sponsorshipThe Mevlana University [ICAD:04.09.2015/150701024] Funding Source: Medlineen_US
dc.identifier.doi10.1002/jcla.22459
dc.identifier.issn0887-8013
dc.identifier.issn1098-2825
dc.identifier.issue7en_US
dc.identifier.pmid29701251en_US
dc.identifier.scopus2-s2.0-85053462736en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1002/jcla.22459
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10578
dc.identifier.volume32en_US
dc.identifier.wosWOS:000444952700020en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Clinical Laboratory Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntibiotic Susceptibilityen_US
dc.subjectDendrogram Analysisen_US
dc.subjectHplcen_US
dc.subjectMycobacteriaen_US
dc.subjectMycobacterium Tuberculosisen_US
dc.titleDetermination of antibiotic resistance and high-performance liquid chromatography profiles for Mycobacterium speciesen_US
dc.typeArticleen_US

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