Experimental Modeling of Flavonoid-Biomembrane Interactions

Basit Öğe Kaydı
dc.contributor.authorSanver, Didem
dc.contributor.authorMurray, Brent S.
dc.contributor.authorSadeghpour, Amin
dc.contributor.authorRappolt, Michael
dc.contributor.authorNelson, Andrew L.
dc.date.accessioned2024-02-23T14:16:35Z
dc.date.available2024-02-23T14:16:35Z
dc.date.issued2016
dc.departmentNEÜen_US
dc.description.abstractNonspecific interactions of flavonoids with lipids can alter the membrane's features (e.g., thickness and fluctuations) as well as influence their therapeutic potentials. However, relatively little is known about the details of how flavonoids interact with lipid components. Structure-dependent interactions of a variety of flavonoids with phospholipid monolayers on a mercury (Hg) film electrode were established by rapid cyclic voltammetry (RCV). The data revealed that flavonoids adopting a planar configuration altered the membrane properties more significantly than nonplanar flavonoids. Quercetin, rutin, and tiliroside were selected for follow-up experiments with Langmuir monolayers, Brewster angle microscopy (BAM), and small-angle X-ray scattering (SAXS). Relaxation phenomena in DOPC monolayers and visualization of the surface with BAM revealed a pronounced monolayer stabilization effect with both quercetin and tiliroside, whereas rutin disrupted the monolayer structure rendering the surface entirely smooth. SAXS showed a monotonous membrane thinning for all compounds studied associated with an increase in the mean fluctuations of the membrane. Rutin, quercetin, and tiliroside decreased the bilayer thickness of DOPC by similar to 0.45, 0.8, and 1.1 angstrom at 6 mol %, respectively. In addition to the novelty of using lipid monolayers to systematically characterize the structure-activity relationship (SAR) of a variety of flavonoids, this is the first report investigating the effect of tiliroside with biomimetic membrane models. All the flavonoids studied are believed to be localized in the lipid/water interface region. Both this localization and the membrane perturbations have implications for their therapeutic activity.en_US
dc.description.sponsorshipEngineering and Physical Sciences Research Council [EP/H502343/1, EP/G015325/1] Funding Source: researchfish; Natural Environment Research Council [NE/M021378/1, NE/K00686X/1, NE/K001396/1, NE/F011830/1] Funding Source: researchfish; EPSRC [EP/G015325/1, EP/H502343/1] Funding Source: UKRI; NERC [NE/F011830/1, NE/K001396/1, NE/K00686X/1, NE/M021378/1] Funding Source: UKRIen_US
dc.identifier.doi10.1021/acs.langmuir.6b02219
dc.identifier.endpage13243en_US
dc.identifier.issn0743-7463
dc.identifier.issue49en_US
dc.identifier.pmid27951697en_US
dc.identifier.scopus2-s2.0-85006100755en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage13234en_US
dc.identifier.urihttps://doi.org/10.1021/acs.langmuir.6b02219
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12726
dc.identifier.volume32en_US
dc.identifier.wosWOS:000389866300031en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofLangmuiren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleExperimental Modeling of Flavonoid-Biomembrane Interactionsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu