Value of multiplex PCR to determine the bacterial and viral aetiology of pneumonia in school-age children

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dc.contributor.authorAydemir, Yusuf
dc.contributor.authorAydemir, Ozlem
dc.contributor.authorPekcan, Sevgi
dc.contributor.authorOzdemir, Mehmet
dc.date.accessioned2024-02-23T14:20:44Z
dc.date.available2024-02-23T14:20:44Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractBackground: Conventional methods for the aetiological diagnosis of community-acquired pneumonia (CAP) are often insufficient owing to low sensitivity and the long wait for the results of culture and particularly serology, and it often these methods establish a diagnosis in only half of cases. Aim: To evaluate the most common bacterial and viral agents in CAP using a fast responsive PCR method and investigate the relationship between clinical/laboratory features and aetiology, thereby contributing to empirical antibiotic selection and reduction of treatment failure. Methods: In children aged 4-15 years consecutively admitted with a diagnosis of CAP, the 10 most commonly detected bacterial and 12 most commonly detected viral agents were investigated by induced sputum using bacterial culture and multiplex PCR methods. Clinical and laboratory features were compared between bacterial and viral pneumonia. Results: In 78 patients, at least one virus was detected in 38 (48.7%) and at least one bacterium in 32 (41%). In addition, both bacteria and viruses were detected in 16 (20.5%) patients. Overall, the agent detection rate was 69.2%. The most common viruses were respiratory syncytial virus and influenza and the most frequently detected bacteria were S. pneumoniae and H. influenzae. PCR was superior to culture for bacterial isolation (41% vs 13%, respectively). Fever, wheezing and radiological features were not helpful in differentiating between bacterial and viral CAP. White blood cell count, CRP and ESR values were significantly higher in the bacterial/mixed aetiology group than in the viral aetiology group. Conclusion: In CAP, multiplex PCR is highly reliable, superior in detecting multiple pathogens and rapidly identifies aetiological agents. Clinical features are poor for differentiation between bacterial and viral infections. The use of PCR methods allow physicians to provide more appropriate antimicrobial therapy, resulting in a better response to treatment, and it may be possible for use as a routine service if costs can be reduced.en_US
dc.identifier.doi10.1080/20469047.2015.1106080
dc.identifier.endpage34en_US
dc.identifier.issn2046-9047
dc.identifier.issn2046-9055
dc.identifier.issue1en_US
dc.identifier.pmid26750616en_US
dc.identifier.scopus2-s2.0-84978472474en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage29en_US
dc.identifier.urihttps://doi.org/10.1080/20469047.2015.1106080
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13278
dc.identifier.volume37en_US
dc.identifier.wosWOS:000395629100005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofPaediatrics And International Child Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPneumoniaen_US
dc.subjectClinical Featuresen_US
dc.subjectLaboratory Featuresen_US
dc.subjectPcren_US
dc.titleValue of multiplex PCR to determine the bacterial and viral aetiology of pneumonia in school-age childrenen_US
dc.typeArticleen_US

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