Hydrogen and poly-?-hydroxybutyric acid production at various acetate concentrations using Rhodobacter capsulatus DSM 1710

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dc.contributor.authorDemiriz, Burcu Ozsoy
dc.contributor.authorKars, Gokhan
dc.contributor.authorYucel, Meral
dc.contributor.authorEroglu, Inci
dc.contributor.authorGunduz, Ufuk
dc.date.accessioned2024-02-23T14:03:11Z
dc.date.available2024-02-23T14:03:11Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractIn the present study, Rhodobacter capsulatus DSM 1710 was cultured in media having various concentrations of acetate (10-65 mM) to investigate simultaneous production of hydrogen and PHB. The highest cumulative hydrogen productions were observed using 50 mM (11.7 mmol H-2) and 65 mM (11.4 mmol H-2) acetate. However, molar productivity values were higher at 25 mM (0.50 mmol/Lc.h) and 50 mM (0.44 mmol/Lc.h) compared to 65 mM (0.35 mmol/Lc.h). Furthermore, the highest PHB production (234.7 mg/L) was attained using 65 mM acetate. At molecular level, the expressions of nifD and phaC genes were analyzed by reverse transcription-polymerase chain reaction using specifically designed tagged primers. Increasing acetate concentrations resulted in an increase in nifD and phaC expressions. Accordingly, the highest transcription levels were obtained at 65 mM acetate. The kinetic studies on hydrogen production and acetate consumption showed that hydrogen production data was in agreement with the Modified Gompertz Model and acetate was consumed through first order kinetics. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipEuropean Union 6th Framework Project HYVOLUTION [019825]; Middle East Technical University, Turkey [BAP -07-02-2010-00-01]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial supports from European Union 6th Framework Project HYVOLUTION (Contract No: 019825) and Middle East Technical University (BAP -07-02-2010-00-01), Turkey.en_US
dc.identifier.doi10.1016/j.ijhydene.2019.02.036
dc.identifier.endpage17277en_US
dc.identifier.issn0360-3199
dc.identifier.issn1879-3487
dc.identifier.issue32en_US
dc.identifier.scopus2-s2.0-85061994271en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage17269en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijhydene.2019.02.036
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12005
dc.identifier.volume44en_US
dc.identifier.wosWOS:000473117500008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofInternational Journal Of Hydrogen Energyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcetateen_US
dc.subjectPoly-Beta-Hydroxybutyric Aciden_US
dc.subjectGene Expressionen_US
dc.subjectRhodobacter Capsulatusen_US
dc.subjectHydrogenen_US
dc.titleHydrogen and poly-?-hydroxybutyric acid production at various acetate concentrations using Rhodobacter capsulatus DSM 1710en_US
dc.typeArticleen_US

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