Relaxivity properties of magnetoferritin: The iron loading effect

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dc.contributor.authorAslan, Tugba Nur
dc.date.accessioned2024-02-23T14:12:33Z
dc.date.available2024-02-23T14:12:33Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractSelf-assembling ferritin protein cages have been used as a template for magnetic iron oxide nanoparticle synthesis within its 8 nm cavity to be explored as a potential magnetic resonance imaging contrast agent. Here in, magnetic nanocores with various iron content were successfully synthesized using recombinant human H-chain ferritin (HFn) by a controlled mineralization reaction. r1 and r2 relaxivities of the synthesized magnetoferritin nanoparticles were measured and the effect of iron loading factor on the r1 and r2 relaxivity was investigated by using a quite large range of 10 different iron loadings per protein cage (500-5000) at 90 MHz and 300 MHz. The sample with the highest iron loading of 5329 Fe/cage has r2 value of 165.2 mM(-1) s(-1) and r1 value of 1.98 mM(-1) s(-1) at 300 MHz. This high r2 value together with a very low protein and iron concentrations (0.03-0.2 mg/mL and 0.15 mM, respectively) renders magnetoferritin very effective T-2 contrast agents. However, r1 values were found to be smaller than literature values suggesting that magnetoferritin may not serve as T-1 contrast agent in MRI. Moreover, magnetoferritin showed an increase in r2 relaxivity with the iron loadings while r1 values have not been affected by the number of Fe atoms loaded as much as r2 values. This result also sheds light on understanding the formation mechanism of iron oxide core and its contribution on relaxation in MRI. (C) 2022, The Society for Biotechnology, Japan. All rights reserved.en_US
dc.description.sponsorshipDouglas Lab., Montana State University, USAen_US
dc.description.sponsorshipThis study was financially supported by Douglas Lab., Montana State University, USA.en_US
dc.identifier.doi10.1016/j.jbiosc.2022.01.005
dc.identifier.endpage480en_US
dc.identifier.issn1389-1723
dc.identifier.issn1347-4421
dc.identifier.issue5en_US
dc.identifier.pmid35277341en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage474en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiosc.2022.01.005
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12107
dc.identifier.volume133en_US
dc.identifier.wosWOS:000788086100009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSoc Bioscience Bioengineering Japanen_US
dc.relation.ispartofJournal Of Bioscience And Bioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectContrast Agenten_US
dc.subjectIron Oxide Nanoparticlesen_US
dc.subjectMagnetoferritinen_US
dc.subjectMagnetic Resonance Imagingen_US
dc.subjectRelaxivityen_US
dc.titleRelaxivity properties of magnetoferritin: The iron loading effecten_US
dc.typeArticleen_US

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