Comparative Evaluation of In-House and Commercial Real-Time PCR Methods for the Detection of the BK and JC Viruses in Clinical Samples

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dc.contributor.authorOzdemir, Mehmet
dc.contributor.authorAyan, Ugur
dc.contributor.authorSevik, Murat
dc.date.accessioned2024-02-23T14:16:59Z
dc.date.available2024-02-23T14:16:59Z
dc.date.issued2020
dc.departmentNEÜen_US
dc.description.abstractAim The two most common human polyomaviruses are the BK (BKV) and JC viruses (JCV). Diseases associated with polyomavirus usually occur in cases of severe cellular immunosuppression. BKV and JCV can cause many diseases, especially if they are reactivated in an immunosuppressed host. The aim of this study is to compare and evaluate the results of real-time polymerase chain reaction (PCR) methods targeting the small and large T gene regions of the viral genome, considering polymorphisms occurring in the viral genome of BKV and JCV. Materials and Methods Urinary specimens of 82 patients were taken from immunosuppressed patient and sent to molecular microbiology laboratory of Meram Medical Faculty. The small t gene was investigated using a commercial kit (LightMix, Roche) by real-time PCR method. Large T gene was investigated by using the optimized in-house real-time PCR method. Sequence analysis was accepted as the standard method. Results BKV positivity was detected in 9 samples and JCV positivity in 61 samples by real-time PCR method specific to small t gene region; BKV positivity in 21 samples and JCV positivity in 67 samples were determined by real-time PCR method specific to the large T gene region. Statistically, there was a significant difference for BKV, but not significant difference for JCV detection between the two methods. Conclusion Different polymorphisms in the target gene regions were responsible for the different outcomes obtained from this study. With this sensitivity and specificity, in-house PCR method which we used is a candidate for routine diagnosis.en_US
dc.identifier.doi10.1055/s-0040-1716603
dc.identifier.endpage83en_US
dc.identifier.issn0974-2727
dc.identifier.issn0974-7826
dc.identifier.issue2en_US
dc.identifier.pmid32905317en_US
dc.identifier.startpage79en_US
dc.identifier.urihttps://doi.org/10.1055/s-0040-1716603
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12869
dc.identifier.volume12en_US
dc.identifier.wosWOS:000565252300001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherThieme Medical Publ Incen_US
dc.relation.ispartofJournal Of Laboratory Physiciansen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBk Virusen_US
dc.subjectJc Virusen_US
dc.subjectReal-Time Pcren_US
dc.titleComparative Evaluation of In-House and Commercial Real-Time PCR Methods for the Detection of the BK and JC Viruses in Clinical Samplesen_US
dc.typeArticleen_US

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