Metal ion-mediated immobilization of catalase on poly(?-caprolactone)/polyethyleneimine electrospun nanofibers

Basit Öğe Kaydı
dc.contributor.authorCetin, Kemal
dc.date.accessioned2024-02-23T11:25:05Z
dc.date.available2024-02-23T11:25:05Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractA common industrial byproduct known as hydrogen peroxide, which is an essential intermediate, is associated with oxidative damage to brain cells and a number of neurodegenerative disorders. Although catalases are enzymes capable of decomposing hydrogen peroxide into water and oxygen, the low stability of free enzymes limits their industrial use. In the current study, poly(e-caprolactone)/polyethyleneimine nanofibers were fabricated using the electrospinning process and catalase was subsequently immobilized via coordination of Cu2+ ions. The characterization of the catalase-immobilized nanofibers and their catalytic activity for hydrogen peroxide decomposition was evaluated by comparing the free catalase. The studies conducted in the pH range of 5.0-8.5 revealed that the catalase-immobilized nanofibers had their highest levels of enzyme activity at pH 7.0. When compared to free catalase, it was shown that the thermal stability and storage stability of catalase-immobilized nanofibers were significantly improved. Catalase-immobilized nanofibers showed significant reusability, where activity was maintained by around 60% after five repeating cycles. The findings showed that catalase-immobilized PCL/PEI-Cu2+ nanofibers had a high potential for various industrial applications as a substitute for the existing techniques.en_US
dc.identifier.doi10.1002/app.54404
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue36en_US
dc.identifier.scopus2-s2.0-85164566603en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/app.54404
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10529
dc.identifier.volume140en_US
dc.identifier.wosWOS:001026008900001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCatalaseen_US
dc.subjectElectrospinningen_US
dc.subjectImmobilizationen_US
dc.subjectNanofibersen_US
dc.subjectPoly(& Epsilon;-Caprolactone)en_US
dc.subjectPolyethyleneimineen_US
dc.titleMetal ion-mediated immobilization of catalase on poly(?-caprolactone)/polyethyleneimine electrospun nanofibersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu