Synthesis and characterization of Ag+-decorated poly(glycidyl methacrylate) microparticle design for the adsorption of nucleic acids

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dc.contributor.authorErol, Kadir
dc.contributor.authorUzunoglu, Aytekin
dc.contributor.authorKose, Kazim
dc.contributor.authorSarica, Busra
dc.contributor.authorAvci, Emre
dc.contributor.authorKose, Dursun A.
dc.date.accessioned2024-02-23T14:12:34Z
dc.date.available2024-02-23T14:12:34Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractIn this study, we report on the adsorption of RNA and DNA molecules by exploiting the high binding affinity of these nucleic adds to Ag+ ions anchored on magnetic poly(glycidyl methacrylate) (PGMA) microparticles. PGMA micropartides were synthesized and modified with nicotinamide which enabled to anchor Ag+ ions on the surface. The successful preparation of PGMA was confirmed by the presence of characteristic FTIR peaks. The ESR results showed that the incorporation of Fe-Ni salt to the polymeric structure provided a magnetic property to the micropartides. The amount of nicotinamide and Ag+ ions used to modify the surface of the particles were found to be 1.79 wt% and 52.6 mg Ag/g microparticle, respectively. The high affinity of nucleic acids to Ag ions were exploited for the adsorption studies. At the optimum working conditions, the adsorption capacity of microparticles was found to be 40.1 and 11.48 mg nucleic acid/g microparticle for RNA and DNA, respectively. Our study indicated that the use of novel Ag+-decorated magnetic PGMA particles can be successfully employed as adsorbents for fast, easy, and cost-friendly adsorption of nucleic acids with high purity as well as high in quantity.en_US
dc.identifier.doi10.1016/j.jchromb.2018.02.017
dc.identifier.endpage11en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid29494983en_US
dc.identifier.scopus2-s2.0-85042483898en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage5en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2018.02.017
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12114
dc.identifier.volume1081en_US
dc.identifier.wosWOS:000430768300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRnaen_US
dc.subjectDnaen_US
dc.subjectAdsorptionen_US
dc.subjectChromatographyen_US
dc.subjectSilveren_US
dc.subjectPoly(Glycidyl Methacrylate)en_US
dc.titleSynthesis and characterization of Ag+-decorated poly(glycidyl methacrylate) microparticle design for the adsorption of nucleic acidsen_US
dc.typeArticleen_US

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